PMID- 23098533
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20190606
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 13
IP  - 8
DP  - 2012
TI  - In vitro biological characterization of DCUN1D5 in DNA damage response.
PG  - 4157-62
AB  - BACKGROUND: Novel prognostic biomarkers or therapeutic molecular targets for 
      laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought 
      to identify multiple novel LSCC-associated genes. METHODS: Using high-density 
      microarray expression profiling, we identified multiple genes that were 
      significantly altered between human LSCCs and paired normal tissues. Potential 
      oncogenic functions of one such gene, DCUN1D5, were further characterized in 
      vitro. RESULTS: Our results demonstrated that DCUN1D5 was highly expressed in 
      LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular 
      migration, 67.5% increased invasive capacity, and 2.6-fold increased 
      proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent 
      manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the 
      number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. 
      CONCLUSION: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA 
      damage response, but further studies are warranted to assess its significance in 
      vivo.
FAU - Guo, Wei
AU  - Guo W
AD  - Department of Otolaryngology-Head and Neck Surgery, Key Laboratory of 
      Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical 
      University, Beijing, China.
FAU - Li, Guo-Jun
AU  - Li GJ
FAU - Xu, Hong-Bo
AU  - Xu HB
FAU - Xie, Jie-Shi
AU  - Xie JS
FAU - Shi, Tai-Ping
AU  - Shi TP
FAU - Zhang, Sheng-Zhong
AU  - Zhang SZ
FAU - Chen, Xiao-Hong
AU  - Chen XH
FAU - Huang, Zhi-Gang
AU  - Huang ZG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 6.3.2.- (DCUN1D5 protein, human)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/*genetics
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Cell Cycle
MH  - Cell Movement
MH  - Cell Proliferation
MH  - DNA Damage/*genetics
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis
MH  - Oncogene Proteins/genetics/*metabolism
MH  - Peptide Synthases/genetics/*metabolism
MH  - Precancerous Conditions
MH  - Prognosis
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Wound Healing
EDAT- 2012/10/27 06:00
MHDA- 2013/04/20 06:00
CRDT- 2012/10/27 06:00
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - 10.7314/apjcp.2012.13.8.4157 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2012;13(8):4157-62. doi: 10.7314/apjcp.2012.13.8.4157.