PMID- 23098564
OWN - NLM
STAT- MEDLINE
DCOM- 20130403
LR  - 20211021
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Print)
IS  - 1021-7770 (Linking)
VI  - 19
IP  - 1
DP  - 2012 Oct 25
TI  - CXC chemokine ligand 12/stromal cell-derived factor-1 regulates cell adhesion in 
      human colon cancer cells by induction of intercellular adhesion molecule-1.
PG  - 91
LID - 10.1186/1423-0127-19-91 [doi]
AB  - BACKGROUND: The CXC chemokine ligand 12 (CXCL12)/stromal cell-derived factor-1 
      (SDF-1) and CXC receptor 4 (CXCR4) axis is involved in human colorectal cancer 
      (CRC) carcinogenesis and can promote the progression of CRC. Interaction between 
      CRC cells and endothelium is a key event in tumor progression. The aim of this 
      study was to investigate the effect of SDF-1 on the adhesion of CRC cells. 
      METHODS: Human CRC DLD-1 cells were used to study the effect of SDF-1 on 
      intercellular adhesion molecule-1 (ICAM-1) expression and cell adhesion to 
      endothelium. RESULTS: SDF-1 treatment induced adhesion of DLD-1 cells to the 
      endothelium and increased the expression level of the ICAM-1. Inhibition of 
      ICAM-1 by small interfering RNA (siRNA) and neutralizing antibody inhibited 
      SDF-1-induced cell adhesion. By using specific inhibitors and short hairpin RNA 
      (shRNA), we demonstrated that the activation of ERK, JNK and p38 pathways is 
      critical for SDF-1-induced ICAM-1 expression and cell adhesion. Promoter activity 
      and transcription factor ELISA assays showed that SDF-1 increased Sp1-, C/EBP-beta- 
      and NF-kappaB-DNA binding activities in DLD-1 cells. Inhibition of Sp1, C/EBP-beta and 
      NF-kappaB activations by specific siRNA blocked the SDF-1-induced ICAM-1 promoter 
      activity and expression. The effect of SDF-1 on cell adhesion was mediated by the 
      CXCR4. CONCLUSION: Our findings support the hypothesis that ICAM-1 up-regulation 
      stimulated by SDF-1 may play an active role in CRC cell adhesion.
FAU - Tung, Shui-Yi
AU  - Tung SY
AD  - Department of Hepato-Gastroenterology, Chang Gung Memorial Hospital, Chiayi, 
      Taiwan.
FAU - Chang, Shun-Fu
AU  - Chang SF
FAU - Chou, Ming-Hui
AU  - Chou MH
FAU - Huang, Wen-Shih
AU  - Huang WS
FAU - Hsieh, Yung-Yu
AU  - Hsieh YY
FAU - Shen, Chien-Heng
AU  - Shen CH
FAU - Kuo, Hsing-Chun
AU  - Kuo HC
FAU - Chen, Cheng-Nan
AU  - Chen CN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121025
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
RN  - 0 (CXCR4 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Receptors, CXCR4)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
EIN - J Biomed Sci. 2013;20:21
MH  - Cell Adhesion/drug effects/*genetics
MH  - Cell Line, Tumor
MH  - *Chemokine CXCL12/genetics/metabolism/pharmacology
MH  - *Colonic Neoplasms/genetics/metabolism
MH  - Endothelium/metabolism/pathology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Receptors, CXCR4/genetics/metabolism
MH  - Signal Transduction
PMC - PMC3488341
EDAT- 2012/10/27 06:00
MHDA- 2013/04/04 06:00
PMCR- 2012/10/25
CRDT- 2012/10/27 06:00
PHST- 2012/07/30 00:00 [received]
PHST- 2012/10/22 00:00 [accepted]
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/04/04 06:00 [medline]
PHST- 2012/10/25 00:00 [pmc-release]
AID - 1423-0127-19-91 [pii]
AID - 10.1186/1423-0127-19-91 [doi]
PST - epublish
SO  - J Biomed Sci. 2012 Oct 25;19(1):91. doi: 10.1186/1423-0127-19-91.