PMID- 23099429
OWN - NLM
STAT- MEDLINE
DCOM- 20130610
LR  - 20211021
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 66
IP  - 2
DP  - 2013 Feb
TI  - Staphylococci isolated from carriage sites and infected sites of dogs as a 
      reservoir of multidrug resistance and methicillin resistance.
PG  - 169-73
LID - 10.1007/s00284-012-0254-9 [doi]
AB  - The aim of this study was to compare the spread of multidrug-resistant (MDR) and 
      methicillin-resistant (MR) staphylococci in healthy dogs and in dogs with evident 
      symptoms of infection. The samples from 172 healthy and 197 infected dogs were 
      examined. The staphylococci were identified with conventional methods and by 
      means of the polymerase chain reaction-restriction fragment length polymorphism 
      (PCR-RFLP) method (MboI). Susceptibility to 15 antibiotics from 10 different 
      antimicrobial classes was tested. Resistance to methicillin was confirmed by the 
      presence of Staphylococcus aureus mecA and S. sciuri mecA genes. Multidrug 
      resistance was defined as resistance to three or more antimicrobial classes. The 
      oral mucosa to be the most frequent site of staphylococcal colonization (55.8 %), 
      followed by nasal cavity (44.2 %), and anus (32.6 %). The prevalence of MDR 
      staphylococci in infected dogs was significantly higher than in the healthy 
      animals (74/137 vs. 34/95, P = 0.006). The MR strains of S. pseudintermedius 
      (2.9 %) originated solely from infected dogs. In contrast, the MR 
      coagulase-negative strains (7.4 %) were isolated solely from healthy dogs. S. 
      aureus strains originated from nasal swabs, MRSA strains were not isolated. MDR 
      staphylococci and MR S. pseudintermedius are more common among infected dogs, but 
      coagulase-negative staphylococci (mostly S. sciuri) seem to be a reservoir of 
      methicillin resistance in healthy dogs.
FAU - Garbacz, Katarzyna
AU  - Garbacz K
AD  - Department of Medical Microbiology, Medical University of Gdansk, ul. Do 
      Studzienki 38, 80-227, Gdansk, Poland. katarzyna.garbacz@scipro.pl
FAU - Zarnowska, Sabina
AU  - Zarnowska S
FAU - Piechowicz, Lidia
AU  - Piechowicz L
FAU - Haras, Krystyna
AU  - Haras K
LA  - eng
PT  - Journal Article
DEP - 20121026
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Carrier State/*microbiology
MH  - DNA, Bacterial/genetics
MH  - Disease Reservoirs
MH  - Dog Diseases/*microbiology
MH  - Dogs
MH  - Drug Resistance, Multiple, Bacterial
MH  - Microbial Sensitivity Tests
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Staphylococcal Infections/microbiology/*veterinary
MH  - Staphylococcus/classification/*drug effects/genetics/*isolation & purification
EDAT- 2012/10/27 06:00
MHDA- 2013/06/12 06:00
CRDT- 2012/10/27 06:00
PHST- 2012/08/23 00:00 [received]
PHST- 2012/10/03 00:00 [accepted]
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - 10.1007/s00284-012-0254-9 [doi]
PST - ppublish
SO  - Curr Microbiol. 2013 Feb;66(2):169-73. doi: 10.1007/s00284-012-0254-9. Epub 2012 
      Oct 26.