PMID- 23099565
OWN - NLM
STAT- MEDLINE
DCOM- 20131126
LR  - 20190111
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 137
IP  - 24
DP  - 2012 Dec 21
TI  - Multi-component analysis of bile acids in natural Calculus bovis and its 
      substitutes by ultrasound-assisted solid-liquid extraction and UPLC-ELSD.
PG  - 5845-53
LID - 10.1039/c2an36084e [doi]
AB  - An ultrasound-assisted solid-liquid extraction (USLE) coupled to 
      ultra-performance liquid chromatography-evaporative light scattering detection 
      (UPLC-ELSD) method has been developed for the simultaneous extraction and 
      determination of six bile acids (BAs) in natural Calculus bovis and its 
      substitutes, collected from different origins. The USLE conditions, UPLC 
      chromatographic and ELSD conditions for BAs were optimized. Under optimum 
      conditions, the six target analytes were efficiently extracted and baseline 
      separated within 10 min. The limits of detection (LODs) and quantification (LOQs) 
      for six BAs were less than 7 ng and 22 ng, respectively. Average recoveries were 
      within the range of 98.8-100.7% with relative standard deviations (RSDs) <2% for 
      the six analytes. This method, due to its convenience, high selectivity, fast 
      analysis efficiency and good reproducibility can be employed for analyzing the 
      content differences of six BAs in 40 batches of natural C. bovis and its existing 
      substitutes. The differences of the content of each BA in natural C. bovis and 
      its substitutes were significant, and the total contents of six BAs in 13 batches 
      of natural C. bovis were in the range of 7.96-160.17 mg g(-1), in 20 natural C. 
      bovis of 0-245.89 mg g(-1), in 2 artificial cultivated C. bovis of 178.48-194.22 
      mg g(-1), in 3 cultured C. bovis of 41.01-107.3 mg g(-1), and in 2 counterfeit C. 
      bovis of 144.9-340.25 mg g(-1). The significant differences of multi-component 
      contents reflected the various inherent qualities of these samples, so, the use 
      of these substitutes as the replacers of natural source in clinic should be paid 
      more attention. Some substitutes could not be used as the replacers.
FAU - Kong, Wei-Jun
AU  - Kong WJ
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
FAU - Xing, Xiao-Yan
AU  - Xing XY
FAU - Xiao, Xiao-He
AU  - Xiao XH
FAU - Wang, Jia-Bo
AU  - Wang JB
FAU - Zhao, Yan-Ling
AU  - Zhao YL
FAU - Yang, Mei-Hua
AU  - Yang MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Biological Products)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 4162LZ0N4O (niuhuang)
SB  - IM
MH  - Bile Acids and Salts/*analysis/*isolation & purification
MH  - Biological Products
MH  - Chemical Fractionation/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - *Light
MH  - Reproducibility of Results
MH  - Scattering, Radiation
MH  - Time Factors
MH  - *Ultrasonics
MH  - Volatilization
EDAT- 2012/10/27 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/10/27 06:00
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1039/c2an36084e [doi]
PST - ppublish
SO  - Analyst. 2012 Dec 21;137(24):5845-53. doi: 10.1039/c2an36084e.