PMID- 23100233
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20161125
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 952
DP  - 2013
TI  - FISH analysis using PPAR gamma-specific probes for detection of PAX8-PPAR gamma 
      translocation in follicular thyroid neoplasms.
PG  - 187-96
LID - 10.1007/978-1-62703-155-4_13 [doi]
AB  - Fluorescence in situ hybridization (FISH) is increasingly gaining importance in 
      clinical diagnostics settings. Due to the ability of the technique to detect 
      chromosomal abnormalities in samples with low cellularity or containing a mixed 
      population of cells even at a single-cell level, it has become more popular in 
      cancer research and diagnosis. Here, we describe the FISH technique for detection 
      of PAX8-PPARgamma translocation in follicular thyroid neoplasms, and the optimal 
      protocol for the detection of this fusion gene using in archival formalin-fixed 
      paraffin-embedded (FFPE) thyroid tissue sections.
FAU - Sharifah, Noor Akmal Syed Husain
AU  - Sharifah NA
AD  - Department of Pathology, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 
      Bandar Tun Razak, Kuala Lumpur, Malaysia, sharifah@ppukm.ukm.my.
FAU - Zakaria, Zubaidah
AU  - Zakaria Z
FAU - Chia, Wai Kit
AU  - Chia WK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 0 (PAX8 Transcription Factor)
RN  - 0 (PAX8 protein, human)
RN  - 0 (PPAR gamma)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 47165-04-8 (DAPI)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Adenocarcinoma, Follicular/*metabolism/pathology
MH  - Chemical Precipitation
MH  - DNA Probes/chemistry/*metabolism
MH  - Filtration
MH  - Fluorescent Dyes/chemistry
MH  - Formaldehyde/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles/metabolism
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Denaturation
MH  - PAX8 Transcription Factor
MH  - PPAR gamma/genetics/*metabolism
MH  - Paired Box Transcription Factors/*genetics
MH  - Paraffin Embedding
MH  - Pepsin A/metabolism
MH  - Protein Transport
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Tissue Fixation
EDAT- 2012/10/27 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/10/27 06:00
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 10.1007/978-1-62703-155-4_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;952:187-96. doi: 10.1007/978-1-62703-155-4_13.