PMID- 23100511
OWN - NLM
STAT- MEDLINE
DCOM- 20130118
LR  - 20211021
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 189
IP  - 11
DP  - 2012 Dec 1
TI  - ATP-binding cassette transporter G1 intrinsically regulates invariant NKT cell 
      development.
PG  - 5129-38
LID - 10.4049/jimmunol.1201570 [doi]
AB  - ATP-binding cassette transporter G1 (ABCG1) plays a role in the intracellular 
      transport of cholesterol. Invariant NKT (iNKT) cells are a subpopulation of T 
      lymphocytes that recognize glycolipid Ags. In this study, we demonstrate that 
      ABCG1 regulates iNKT cell development and functions in a cell-intrinsic manner. 
      Abcg1(-/-) mice displayed reduced frequencies of iNKT cells in thymus and 
      periphery. Thymic iNKT cells deficient in ABCG1 had reduced membrane lipid raft 
      content, and showed impaired proliferation and defective maturation during the 
      early stages of development. Moreover, we found that Abcg1(-/-) mice possess a 
      higher frequency of Vbeta7(+) iNKT cells, suggesting alterations in iNKT cell thymic 
      selection. Furthermore, in response to CD3epsilon/CD28 stimulation, Abcg1(-/-) thymic 
      iNKT cells showed reduced production of IL-4 but increased production of IFN-gamma. 
      Our results demonstrate that changes in intracellular cholesterol homeostasis by 
      ABCG1 profoundly impact iNKT cell development and function.
FAU - Sag, Duygu
AU  - Sag D
AD  - Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 
      La Jolla, CA 92037, USA.
FAU - Wingender, Gerhard
AU  - Wingender G
FAU - Nowyhed, Heba
AU  - Nowyhed H
FAU - Wu, Runpei
AU  - Wu R
FAU - Gebre, Abraham K
AU  - Gebre AK
FAU - Parks, John S
AU  - Parks JS
FAU - Kronenberg, Mitchell
AU  - Kronenberg M
FAU - Hedrick, Catherine C
AU  - Hedrick CC
LA  - eng
GR  - R01 HL094525/HL/NHLBI NIH HHS/United States
GR  - R01 AI045053/AI/NIAID NIH HHS/United States
GR  - R01 AI4505/AI/NIAID NIH HHS/United States
GR  - R37 AI071922/AI/NIAID NIH HHS/United States
GR  - P01 HL055798/HL/NHLBI NIH HHS/United States
GR  - R01 HL097368/HL/NHLBI NIH HHS/United States
GR  - S10 RR027366/RR/NCRR NIH HHS/United States
GR  - R37 AI71922/AI/NIAID NIH HHS/United States
GR  - R01 HL085790/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121024
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antibodies)
RN  - 0 (CD28 Antigens)
RN  - 0 (CD3 Complex)
RN  - 0 (Lipoproteins)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*immunology
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Biological Transport/genetics/immunology
MH  - CD28 Antigens/agonists/immunology
MH  - CD3 Complex/immunology
MH  - Cell Differentiation/genetics/immunology
MH  - Cell Proliferation
MH  - Cholesterol/*immunology/metabolism
MH  - Gene Expression Regulation/*immunology
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-4/biosynthesis/immunology
MH  - Lipoproteins/genetics/*immunology
MH  - Membrane Microdomains/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Natural Killer T-Cells/cytology/*immunology
MH  - Primary Cell Culture
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics/immunology
MH  - *Signal Transduction
MH  - Thymus Gland
PMC - PMC3776580
MID - NIHMS511813
EDAT- 2012/10/27 06:00
MHDA- 2013/01/19 06:00
PMCR- 2013/12/01
CRDT- 2012/10/27 06:00
PHST- 2012/10/27 06:00 [entrez]
PHST- 2012/10/27 06:00 [pubmed]
PHST- 2013/01/19 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - jimmunol.1201570 [pii]
AID - 10.4049/jimmunol.1201570 [doi]
PST - ppublish
SO  - J Immunol. 2012 Dec 1;189(11):5129-38. doi: 10.4049/jimmunol.1201570. Epub 2012 
      Oct 24.