PMID- 23100654
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121029
LR  - 20211021
IS  - 0046-8991 (Print)
IS  - 0973-7715 (Electronic)
IS  - 0046-8991 (Linking)
VI  - 47
IP  - 2
DP  - 2007 Jun
TI  - Glucose dehydrogenase of a rhizobacterial strain of Enterobacter asburiae 
      involved in mineral phosphate solubilization shares properties and sequence 
      homology with other members of enterobacteriaceae.
PG  - 126-31
LID - 10.1007/s12088-007-0025-7 [doi]
AB  - Glucose dehydrogenase (GDH) of Gram-negative bacteria is a membrane bound enzyme 
      catalyzing the oxidation of glucose to gluconic acid and is involved in the 
      solubilization of insoluble mineral phosphate complexes. A 2.4 kb glucose 
      dehydrogenase gene (gcd) of Enterobacter asburiae sharing extensive homology to 
      the gcd of other enterobacteriaceae members was cloned in a PCR-based directional 
      genome walking approach and the expression confirmed in Escherichia coli YU423 on 
      both MacConkey glucose agar and hydroxyapatite (HAP) containing media. Mineral 
      phosphate solubilization by the cloned E. asburiae gcd was confirmed by the 
      release of significant amount of phosphate in HAP containing liquid medium. gcd 
      was over expressed in E. coli AT15 (gcd::cm) and the purified recombinant protein 
      had a high affinity to glucose, and oxidized galactose and maltose with lower 
      affinities.The enzyme was highly sensitive to heat and EDTA, and belonged to Type 
      I, similar to GDH of E. coli.
FAU - Tripura, C
AU  - Tripura C
AD  - Department of Plant Sciences, University of Hyderabad, P. O. Central University, 
      Hyderabad, 500 046 Andhra Pradesh India.
FAU - Sudhakar Reddy, P
AU  - Sudhakar Reddy P
FAU - Reddy, M K
AU  - Reddy MK
FAU - Sashidhar, B
AU  - Sashidhar B
FAU - Podile, A R
AU  - Podile AR
LA  - eng
PT  - Journal Article
DEP - 20070708
PL  - India
TA  - Indian J Microbiol
JT  - Indian journal of microbiology
JID - 0374703
PMC - PMC3450103
OTO - NOTNLM
OT  - Enterobacter asburiae
OT  - Glucose dehydrogenase
OT  - Mineral phosphate solubilization
EDAT- 2007/06/01 00:00
MHDA- 2007/06/01 00:01
PMCR- 2008/06/01
CRDT- 2012/10/27 06:00
PHST- 2007/03/15 00:00 [received]
PHST- 2007/03/24 00:00 [revised]
PHST- 2007/03/30 00:00 [accepted]
PHST- 2012/10/27 06:00 [entrez]
PHST- 2007/06/01 00:00 [pubmed]
PHST- 2007/06/01 00:01 [medline]
PHST- 2008/06/01 00:00 [pmc-release]
AID - 25 [pii]
AID - 10.1007/s12088-007-0025-7 [doi]
PST - ppublish
SO  - Indian J Microbiol. 2007 Jun;47(2):126-31. doi: 10.1007/s12088-007-0025-7. Epub 
      2007 Jul 8.