PMID- 23101512
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20190608
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Linking)
VI  - 45
IP  - 10
DP  - 2012 Oct
TI  - Mesodermal repression of single-minded in Drosophila embryo is mediated by a 
      cluster of Snail-binding sites proximal to the early promoter.
PG  - 577-82
AB  - single-minded (sim) is a master regulatory gene that directs differentiation in 
      the central nervous system during Drosophila embryogenesis. Recent identification 
      of the mesectoderm enhancer (MSE) of sim has led to the hypothesis that two Snail 
      (Sna)-binding sites in the MSE may repress sim expression in the presumptive 
      mesoderm. We provide evidence here that three Sna-binding sites proximal to the 
      sim promoter, but not those of the MSE, are responsible for the mesodermal 
      repression of sim in vivo. Using transgenic embryos injected with lacZ 
      transgenes, we showed that sim repression in the mesoderm requires the three 
      promoter-proximal Sna-binding sites. These results suggest that Sna represses the 
      mesectodermal expression of sim by directly repressing the nearby promoter, and 
      not by quenching adjacent transcriptional activators in the MSE. These data also 
      showed how the MSE, lacking the three proximal Sna-binding sites, reproduced the 
      endogenous pattern of sim expression in transgenic embryos.
FAU - Park, Kye Won
AU  - Park KW
AD  - Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 
      Korea.
FAU - Hong, Joung-Woo
AU  - Hong JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Snail Family Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 0 (sim protein, Drosophila)
RN  - 0 (sna protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism
MH  - Binding Sites
MH  - Drosophila/growth & development/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Embryo, Nonmammalian/metabolism
MH  - Mesoderm/*metabolism
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Promoter Regions, Genetic
MH  - Snail Family Transcription Factors
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2012/10/30 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/10/30 06:00
PHST- 2012/10/30 06:00 [entrez]
PHST- 2012/10/30 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 10.5483/bmbrep.2012.45.10.105 [doi]
PST - ppublish
SO  - BMB Rep. 2012 Oct;45(10):577-82. doi: 10.5483/bmbrep.2012.45.10.105.