PMID- 23103637
OWN - NLM
STAT- MEDLINE
DCOM- 20130531
LR  - 20121226
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 415
DP  - 2013 Jan 16
TI  - Detection of autoantibodies against platelet glycoproteins in patients with 
      immune thrombocytopenic purpura by flow cytometric immunobead array.
PG  - 176-80
LID - S0009-8981(12)00502-5 [pii]
LID - 10.1016/j.cca.2012.10.035 [doi]
AB  - BACKGROUND: The goal of this study is to develop a flow cytometric immunobead 
      array (FCIA) assay to detect platelet autoantibodies commonly present in bleeding 
      patients with immune thrombocytopenic purpura (ITP). METHODS: Polystyrene 
      microbeads coated with antibodies against human platelet glycoproteins (GPs) IX 
      (SZ1), Ib (SZ2), IIIa (SZ21), IIb (SZ22), and P-selectin (SZ51) were incubated 
      with platelet lysate from 50 ITP patients and 86 controls. The platelet 
      antigen-autoantibody complexes were detected by flow cytometry using an 
      FITC-labeled antibody. The results were compared with that of a monoclonal 
      antibody immobilization of platelet antigen (MAIPA) assay. RESULTS: By FCIA, 
      platelet autoantibodies against GPIb, GPIIb, GPIIIa, GPIX and P-selectin were 
      detected in ITP patients. Mean fluorescent intensity values with antibodies SZ1, 
      SZ2, SZ21, SZ22 and SZ51 were all higher in ITP patients than controls (p 
      values<0.01). In ROC analysis, values of the area under the curve were 0.89, 
      0.82, 0.93, 0.94 and 0.95, respectively. In ITP diagnosis, the FCIA assay with 
      these five antibodies had better sensitivity and accuracy than the MAIPA assay 
      (96% vs. 44% in sensitivity; 80.9% vs. 64.7% in accuracy, p<0.01). CONCLUSION: 
      FCIA assays with multiple antibodies against platelet GPs may be used to improve 
      the diagnosis of ITP in hospitals.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - He, Yang
AU  - He Y
AD  - Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, the First 
      Affiliated Hospital of Soochow University, Suzhou, China. heyang1963@163.com
FAU - Zhao, Yun-xiao
AU  - Zhao YX
FAU - Zhu, Ming-qing
AU  - Zhu MQ
FAU - Wu, Qingyu
AU  - Wu Q
FAU - Ruan, Chang-geng
AU  - Ruan CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121025
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Polystyrenes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigen-Antibody Complex/*analysis
MH  - Autoantibodies/*analysis
MH  - Autoantigens/chemistry/*immunology
MH  - Blood Platelets/chemistry/*immunology
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Fluorescent Dyes
MH  - Humans
MH  - Immunoassay
MH  - Male
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/chemistry/*immunology
MH  - Polystyrenes
MH  - Purpura, Thrombocytopenic, Idiopathic/*diagnosis/immunology
MH  - ROC Curve
EDAT- 2012/10/30 06:00
MHDA- 2013/06/01 06:00
CRDT- 2012/10/30 06:00
PHST- 2012/09/10 00:00 [received]
PHST- 2012/10/08 00:00 [revised]
PHST- 2012/10/18 00:00 [accepted]
PHST- 2012/10/30 06:00 [entrez]
PHST- 2012/10/30 06:00 [pubmed]
PHST- 2013/06/01 06:00 [medline]
AID - S0009-8981(12)00502-5 [pii]
AID - 10.1016/j.cca.2012.10.035 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2013 Jan 16;415:176-80. doi: 10.1016/j.cca.2012.10.035. Epub 2012 
      Oct 25.