PMID- 23103946
OWN - NLM
STAT- MEDLINE
DCOM- 20131126
LR  - 20121116
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 137
IP  - 24
DP  - 2012 Dec 21
TI  - A peroxidase-active aptazyme as an isothermally amplifiable label in an 
      aptazyme-linked oligonucleotide assay for low-picomolar IgE detection.
PG  - 5710-2
LID - 10.1039/c2an36201e [doi]
AB  - A peroxidase-active hemin-aptamer was clicked onto an immunoglobulin E (IgE) 
      binding aptamer. The resulting bifunctional aptamer was successfully used in a 
      highly sensitive aptazyme-linked oligonucleotide assay (ALONA) for the detection 
      of IgE. The sensitivity of the assay is enhanced by isothermal amplification of 
      the aptazyme followed by colorimetric detection of the aptazyme's peroxidase 
      activity, which can easily be evaluated even without advanced lab instruments.
FAU - Pollet, Jeroen
AU  - Pollet J
AD  - Department of Chemical & Biomolecular Engineering, University of Houston, TX 
      77204, USA.
FAU - Strych, Ulrich
AU  - Strych U
FAU - Willson, Richard C
AU  - Willson RC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Oligonucleotides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 743LRP9S7N (Hemin)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Aptamers, Nucleotide/chemistry/*metabolism
MH  - Biosensing Techniques/*methods
MH  - G-Quadruplexes
MH  - Hemin/metabolism
MH  - Immunoglobulin E/*analysis/metabolism
MH  - Limit of Detection
MH  - Oligonucleotides/*metabolism
MH  - Peroxidase/*metabolism
MH  - Staining and Labeling
MH  - *Temperature
EDAT- 2012/10/30 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/10/30 06:00
PHST- 2012/10/30 06:00 [entrez]
PHST- 2012/10/30 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1039/c2an36201e [doi]
PST - ppublish
SO  - Analyst. 2012 Dec 21;137(24):5710-2. doi: 10.1039/c2an36201e.