PMID- 23107103
OWN - NLM
STAT- MEDLINE
DCOM- 20131028
LR  - 20211021
IS  - 1750-3639 (Electronic)
IS  - 1015-6305 (Print)
IS  - 1015-6305 (Linking)
VI  - 23
IP  - 3
DP  - 2013 May
TI  - Chromogenic in situ hybridization is a reliable alternative to fluorescence in 
      situ hybridization for diagnostic testing of 1p and 19q loss in paraffin-embedded 
      gliomas.
PG  - 311-8
LID - 10.1111/bpa.12003 [doi]
AB  - Recent studies imply the importance of rapid and reliable diagnostic assessment 
      of 1p/19q status in oligodendroglial tumors. To date, fluorescence in situ 
      hybridization (FISH) is the most commonly applied technique. FISH, however, has 
      several technical shortcomings that are suboptimal for diagnostic applications: 
      results must be viewed in a fluorescence microscope, results are usually 
      evaluated by a single investigator only, and signal fading excludes physical 
      archiving. Also, in gliomas, the distinction of diffusely infiltrating tumor 
      cells from reactively altered normal tissue may be challenging in fluorescence 
      microscopy. Dual-color chromogenic in situ hybridization (CISH) has started to 
      replace FISH in some diagnostic tests performed in pathology. Here, we present 
      the first single institute experience with a side-by-side analysis of 1p/19q FISH 
      and CISH in a series of 42 consecutive gliomas. FISH and CISH produced identical 
      results for 1p and 19q in 93% of cases (n = 39/42). Discrepant results were 
      reevaluated by repeated FISH and a polymerase chain reaction (PCR)-based 
      microsatellite marker analysis for loss of heterozygosity. Reevaluation confirmed 
      CISH data in all three cases. We conclude that CISH is a reliable alternative in 
      1p/19q testing in paraffin-embedded tissues likely to be more sensitive to detect 
      1p/19q status than FISH analysis.
CI  - (c) 2012 The Authors; Brain Pathology (c) 2012 International Society of 
      Neuropathology.
FAU - Lass, Ulrike
AU  - Lass U
AD  - Clinical Cooperation Unit Neuropathology, G380, German Cancer Center (DKFZ), 
      Heidelberg, Germany.
FAU - Hartmann, Christian
AU  - Hartmann C
FAU - Capper, David
AU  - Capper D
FAU - Herold-Mende, Christel
AU  - Herold-Mende C
FAU - von Deimling, Andreas
AU  - von Deimling A
FAU - Meiboom, Maren
AU  - Meiboom M
FAU - Mueller, Wolf
AU  - Mueller W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20121129
PL  - Switzerland
TA  - Brain Pathol
JT  - Brain pathology (Zurich, Switzerland)
JID - 9216781
RN  - 0 (Fixatives)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Neoplasms/*diagnosis/*pathology
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Female
MH  - Fixatives
MH  - Formaldehyde
MH  - Glioma/*diagnosis/*pathology
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Loss of Heterozygosity
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Young Adult
PMC - PMC8029485
EDAT- 2012/10/31 06:00
MHDA- 2013/10/29 06:00
PMCR- 2012/11/29
CRDT- 2012/10/31 06:00
PHST- 2012/10/02 00:00 [received]
PHST- 2012/10/18 00:00 [accepted]
PHST- 2012/10/31 06:00 [entrez]
PHST- 2012/10/31 06:00 [pubmed]
PHST- 2013/10/29 06:00 [medline]
PHST- 2012/11/29 00:00 [pmc-release]
AID - BPA12003 [pii]
AID - 10.1111/bpa.12003 [doi]
PST - ppublish
SO  - Brain Pathol. 2013 May;23(3):311-8. doi: 10.1111/bpa.12003. Epub 2012 Nov 29.