PMID- 23107137
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20161125
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 753
DP  - 2012 Nov 13
TI  - Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in 
      sediment samples by combining subcritical water extraction and dispersive 
      liquid-liquid microextraction with derivatization.
PG  - 57-63
LID - S0003-2670(12)01355-4 [pii]
LID - 10.1016/j.aca.2012.09.028 [doi]
AB  - A sample preparation method for the determination of hydroxylated polycyclic 
      aromatic hydrocarbons (OH-PAHs) in sediment samples was developed using gas 
      chromatography-mass spectrometry (GC-MS). Dispersive liquid-liquid 
      microextraction (DLLME) with derivatization was performed following the 
      subcritical water extraction (SWE) that provided which was provided by 
      accelerated solvent extraction (ASE). Several important parameters that affected 
      both SWE extraction and DLLME, such as the selection of organic modifier, its 
      volume, extraction temperature, extraction pressure and extraction time were also 
      investigated. High sensitivity of the hydroxylated PAHs derivatives by 
      N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) could be 
      achieved with the limits of detection (LODs) ranging from 0.0139 (2-OH-nap) to 
      0.2334 mug kg(-1) (3-OH-fluo) and the relative standard deviations (RSDs) between 
      2.81% (2-OH-phe) and 11.07% (1-OH-pyr). Moreover, the proposed method was 
      compared with SWE coupled with solid phase extraction (SPE), and the results 
      showed that ASE-DLLME was more promising with recoveries ranging from 57.63% to 
      91.07%. The proposed method was then applied to determine the hydroxylated 
      metabolites of phenanthrene in contaminated sediments produced during the 
      degradation by two PAH-degraders isolated from mangrove sediments.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Wang, Xiaowei
AU  - Wang X
AD  - MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun 
      Yat-Sen University, Guangzhou 510275, People's Republic of China.
FAU - Lin, Li
AU  - Lin L
FAU - Luan, Tiangang
AU  - Luan T
FAU - Yang, Lihua
AU  - Yang L
FAU - Tam, Nora F Y
AU  - Tam NF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121001
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Acetamides)
RN  - 0 (Acetonitriles)
RN  - 0 (Fluoroacetates)
RN  - 0 (Organosilicon Compounds)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
RN  - 059QF0KO0R (Water)
RN  - 77377-52-7 (N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetamides/chemistry
MH  - Acetonitriles/chemistry
MH  - Fluoroacetates/chemistry
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Geologic Sediments/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Liquid Phase Microextraction
MH  - Organosilicon Compounds/chemistry
MH  - Polycyclic Aromatic Hydrocarbons/*analysis/isolation & purification/metabolism
MH  - Pressure
MH  - Solid Phase Extraction
MH  - Temperature
MH  - Time Factors
MH  - Water/*chemistry
MH  - Water Pollutants, Chemical/*analysis/isolation & purification/metabolism
EDAT- 2012/10/31 06:00
MHDA- 2013/03/26 06:00
CRDT- 2012/10/31 06:00
PHST- 2012/06/23 00:00 [received]
PHST- 2012/09/12 00:00 [revised]
PHST- 2012/09/14 00:00 [accepted]
PHST- 2012/10/31 06:00 [entrez]
PHST- 2012/10/31 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
AID - S0003-2670(12)01355-4 [pii]
AID - 10.1016/j.aca.2012.09.028 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2012 Nov 13;753:57-63. doi: 10.1016/j.aca.2012.09.028. Epub 2012 
      Oct 1.