PMID- 23109057
OWN - NLM
STAT- MEDLINE
DCOM- 20131114
LR  - 20211021
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 54
IP  - 1
DP  - 2013 Feb
TI  - Molecular characterization, expression profiles, and association analysis with 
      hematologic parameters of the porcine HPSE and HPSE2 genes.
PG  - 71-8
LID - 10.1007/s13353-012-0119-8 [doi]
AB  - Heparan sulfate (HS), which consists of repeating disaccharide units, plays an 
      essential role in inflammation and viral infections. Heparanase (encoded by the 
      HPSE gene) can cleave the HS chains of heparan sulfate proteoglycans (HSPGs), 
      which are known to be important participants in immune responses. HPSE2 
      (heparanase 2) is a homologous gene of HPSE. To investigate the functions of HS, 
      which is the primary receptor of the porcine reproductive and respiratory 
      syndrome virus (PRRSV), the two genes involved in the metabolic process of HS 
      were studied. Here, we present a study of tissue expression profiles, 
      polymorphisms of the HPSE and HPSE2 genes, and the changes of their mRNA levels 
      in porcine alveolar macrophages (PAMs) induced by PRRSV. Both genes are 
      preferentially expressed in porcine immune or immune-related organs under normal 
      conditions, e.g., in the lung, spleen, and lymph node. Moreover, a synonymous 
      mutation c.750A>G located in exon5 of the HPSE gene was detected, and was 
      significantly associated with the white blood cell (WBC) count, red blood cell 
      (RBC) count, hemoglobin (HGB), and hematocrit (HCT) in the peripheral blood 
      (p < 0.05). A single nucleotide polymorphism (SNP) c.2073A>G was found in the 
      HPSE2 gene and association analysis showed that it was significantly associated 
      with the WBC content in the blood (p < 0.05). Upon stimulation in healthy piglets 
      with PRRSV, the HPSE mRNA was obviously up-regulated, while the HPSE2 mRNA did 
      not induce a prominent change in PAMs.
FAU - Tao, Cong
AU  - Tao C
AD  - Laboratory of Molecular Biology and Animal Breeding, Key Laboratory of 
      Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, 
      Huazhong Agricultural University, Wuhan, 430070, China.
FAU - Wang, Weimin
AU  - Wang W
FAU - Zhou, Ping
AU  - Zhou P
FAU - Xia, Tian
AU  - Xia T
FAU - Zhou, Xiang
AU  - Zhou X
FAU - Zeng, Cuiping
AU  - Zeng C
FAU - Zhang, Qingde
AU  - Zhang Q
FAU - Liu, Bang
AU  - Liu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121030
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Virus)
RN  - 9050-30-0 (Heparitin Sulfate)
RN  - EC 3.2.1.- (heparanase)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Erythrocyte Count
MH  - Gene Expression Profiling/*veterinary
MH  - Glucuronidase/*genetics
MH  - Heparan Sulfate Proteoglycans/metabolism
MH  - Heparitin Sulfate/metabolism
MH  - Leukocyte Count
MH  - Macrophages, Alveolar/metabolism/virology
MH  - Molecular Sequence Data
MH  - Polymorphism, Single Nucleotide
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*genetics/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - RNA, Messenger/analysis
MH  - Receptors, Virus/genetics
MH  - Sequence Alignment
MH  - Swine/*genetics
MH  - Transcriptome/*genetics
EDAT- 2012/10/31 06:00
MHDA- 2013/11/15 06:00
CRDT- 2012/10/31 06:00
PHST- 2012/03/07 00:00 [received]
PHST- 2012/10/08 00:00 [accepted]
PHST- 2012/07/06 00:00 [revised]
PHST- 2012/10/31 06:00 [entrez]
PHST- 2012/10/31 06:00 [pubmed]
PHST- 2013/11/15 06:00 [medline]
AID - 10.1007/s13353-012-0119-8 [doi]
PST - ppublish
SO  - J Appl Genet. 2013 Feb;54(1):71-8. doi: 10.1007/s13353-012-0119-8. Epub 2012 Oct 
      30.