PMID- 23110691
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20231104
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 10
DP  - 2012 Oct 30
TI  - Steroidogenic capacity of the placenta as a supplemental source of progesterone 
      during pregnancy in domestic cats.
PG  - 89
LID - 10.1186/1477-7827-10-89 [doi]
AB  - BACKGROUND: Until recently, the corpus luteum (CL) was considered to be the main 
      source of progesterone (P4) during pregnancy in the domestic cat (Felis catus). 
      However, other possible sources of P4 have not been ruled out. Although feline 
      placental homogenates were found to be capable of synthesizing P4, expression of 
      the respective steroidogenic enzymes has not been investigated at the molecular 
      level. Therefore, in the present study, expression of the two major factors 
      involved in the synthesis of P4 - 3beta-hydroxysteroid dehydrogenase (3betaHSD) 
      and steroidogenic acute regulatory protein (StAR) - was investigated in the 
      feline CL and placenta during the course of pseudopregnancy and pregnancy. 
      METHODS: The mRNA levels of StAR and 3betaHSD were determined using Real Time PCR 
      and their localizations were determined by immunohistochemistry. Placental P4 
      concentrations, after ethyl extraction, were measured by EIA. RESULTS: Luteal 
      3betaHSD and StAR mRNA levels were strongly time-dependent, peaking during 
      mid-pregnancy. The placental 3betaHSD mRNA level was significantly upregulated 
      towards the end of pregnancy. In the CL, 3betaHSD and StAR protein were localized 
      in the luteal cells whereas in the placenta they were localized to the maternal 
      decidual cells. Placental P4 concentrations were low in early pregnant queens, 
      but increased along with gestational age. CONCLUSIONS: These results confirm that 
      the placenta is an additional source of P4 in pregnant queens and can thereby be 
      considered as an important endocrine organ supporting feline pregnancy.
FAU - Siemieniuch, Marta J
AU  - Siemieniuch MJ
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn 10-747, 
      Tuwima-St, 10, Poland. m.siemieniuch@pan.olsztyn.pl
FAU - Jursza, Ewelina
AU  - Jursza E
FAU - Szostek, Anna Z
AU  - Szostek AZ
FAU - Skarzynski, Dariusz J
AU  - Skarzynski DJ
FAU - Boos, Alois
AU  - Boos A
FAU - Kowalewski, Mariusz P
AU  - Kowalewski MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121030
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Biomarkers)
RN  - 0 (Phosphoproteins)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/*biosynthesis/genetics
MH  - Animals
MH  - Biomarkers/blood/metabolism
MH  - Cats
MH  - Corpus Luteum/metabolism
MH  - Female
MH  - Phosphoproteins/*biosynthesis
MH  - Placenta/blood supply/*metabolism
MH  - Pregnancy
MH  - Pregnancy, Animal/blood/*metabolism
MH  - Progesterone/blood/*metabolism
MH  - Up-Regulation/physiology
PMC - PMC3522032
EDAT- 2012/11/01 06:00
MHDA- 2013/09/24 06:00
PMCR- 2012/10/30
CRDT- 2012/11/01 06:00
PHST- 2012/03/12 00:00 [received]
PHST- 2012/08/17 00:00 [accepted]
PHST- 2012/11/01 06:00 [entrez]
PHST- 2012/11/01 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
PHST- 2012/10/30 00:00 [pmc-release]
AID - 1477-7827-10-89 [pii]
AID - 10.1186/1477-7827-10-89 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2012 Oct 30;10:89. doi: 10.1186/1477-7827-10-89.