PMID- 23111282
OWN - NLM
STAT- MEDLINE
DCOM- 20130820
LR  - 20141120
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 27
IP  - 2
DP  - 2013 Mar
TI  - Analysis of gene expression changes to elucidate the mechanism of chilling injury 
      in precision-cut liver slices.
PG  - 890-9
LID - S0887-2333(12)00285-8 [pii]
LID - 10.1016/j.tiv.2012.10.009 [doi]
AB  - The exact mechanism of chilling injury (by a decrease of temperature to 
      sub-physiological values), especially in the intact organ, is yet unknown. 
      Precision-cut liver slices (PCLS), which closely resemble the organ from which 
      they are derived, are an ideal in vitro model to study the mechanism of chilling 
      injury in the intact organ. In the present study we were able to separate 
      chilling injury from other damaging events such as cryoprotectant toxicity and 
      ice-crystal injury and performed micro-array analysis of regulated genes. Pathway 
      analysis revealed that different stress responses, lipid/fatty acid and 
      cholesterol biosynthesis and metabolism were affected by chilling. This indicates 
      that the cell-membrane might be the primary site and sensor for chilling, which 
      may initiate and amplify downstream intracellular signaling events. Most 
      importantly, we were able to identify gene expression responses from stellate 
      cells and Kupffer cells suggesting the involvement of all liver cell types in the 
      injury. In conclusion, a broad spectrum of previously unknown gene expression 
      changes induced by chilling was identified in the tissue. This is the first 
      report of a systematic investigation on the mechanism of chilling injury in 
      integrated tissue by micro-array analysis under conditions in which other sources 
      of injury are minimal.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Guan, Na
AU  - Guan N
AD  - Division of Pharmacokinetics, Toxicology and Targeting, Department of Pharmacy, 
      University of Groningen, The Netherlands.
FAU - Blomsma, Sylvia A
AU  - Blomsma SA
FAU - Fahy, Gregory M
AU  - Fahy GM
FAU - Groothuis, Geny M M
AU  - Groothuis GM
FAU - de Graaf, Inge A M
AU  - de Graaf IA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121027
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Cryoprotective Agents)
RN  - 63231-63-0 (RNA)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Cold Temperature/*adverse effects
MH  - Cryoprotective Agents/*adverse effects
MH  - Gene Expression Profiling
MH  - In Vitro Techniques
MH  - Liver/*injuries/*metabolism
MH  - Male
MH  - Oligonucleotide Array Sequence Analysis
MH  - Organ Preservation
MH  - RNA/genetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2012/11/01 06:00
MHDA- 2013/08/21 06:00
CRDT- 2012/11/01 06:00
PHST- 2012/06/14 00:00 [received]
PHST- 2012/10/10 00:00 [revised]
PHST- 2012/10/11 00:00 [accepted]
PHST- 2012/11/01 06:00 [entrez]
PHST- 2012/11/01 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0887-2333(12)00285-8 [pii]
AID - 10.1016/j.tiv.2012.10.009 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2013 Mar;27(2):890-9. doi: 10.1016/j.tiv.2012.10.009. Epub 2012 
      Oct 27.