PMID- 23113940
OWN - NLM
STAT- MEDLINE
DCOM- 20131122
LR  - 20130502
IS  - 1475-2670 (Electronic)
IS  - 0007-4853 (Linking)
VI  - 103
IP  - 3
DP  - 2013 Jun
TI  - Infection, growth and maintenance of Wolbachia pipientis in clonal and non-clonal 
      Aedes albopictus cell cultures.
PG  - 251-60
LID - 10.1017/S0007485312000648 [doi]
AB  - Insect cell lines provide useful in vitro models for studying biological systems, 
      including interactions between mosquitoes and obligate intracellular 
      endosymbionts such as Wolbachia pipientis. The Aedes albopictus Aa23 cell line 
      was the first cell line developed to allow examination of Wolbachia infections. 
      However, Wolbachia studies using Aa23 can be complicated by the presence of 
      different cell types in the cell line and the substantial temporal variation in 
      infection level. Two approaches were examined to ameliorate infection 
      variability. In the first approach, multiple Aa23 passaging regimes were tested 
      for an effect on infection variability. Fluorescence in situ hybridization (FISH) 
      staining was used to characterize Wolbachia infection level over time. The 
      results demonstrate an impact of passaging method on Wolbachia infection level, 
      with some methods resulting in loss of infection. None of the passaging methods 
      succeeded in effectively mitigating infection level variation. In a second 
      approach, the clonal C7-10 A. albopictus cell line was infected with Wolbachia 
      from Aa23 cells and Drosophila simulans (Riverside), resulting in cell lines 
      designated C7-10B and C7-10R, respectively. Characterization via FISH staining 
      showed greater stability and uniformity of Wolbachia infection in C7-10R relative 
      to the infection in C7-10B. Characterization of the Aa23, C7-10B and C7-10R lines 
      is discussed as a tool for the study of Wolbachia-host cell interactions.
FAU - Khoo, C C H
AU  - Khoo CC
AD  - Department of Entomology, University of Kentucky, Lexington, KY 40546, USA. 
      ckhoo@colostate.edu
FAU - Venard, C M P
AU  - Venard CM
FAU - Fu, Y
AU  - Fu Y
FAU - Mercer, D R
AU  - Mercer DR
FAU - Dobson, S L
AU  - Dobson SL
LA  - eng
GR  - R01-AI051533/AI/NIAID NIH HHS/United States
GR  - R01-AI067434/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121101
PL  - England
TA  - Bull Entomol Res
JT  - Bulletin of entomological research
JID - 2984715R
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Aedes/*cytology/*microbiology
MH  - Animals
MH  - Bacterial Proteins/genetics
MH  - Cell Culture Techniques/methods
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - Drosophila/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Polymerase Chain Reaction
MH  - Wolbachia/*genetics/*growth & development
EDAT- 2012/11/02 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S0007485312000648 [pii]
AID - 10.1017/S0007485312000648 [doi]
PST - ppublish
SO  - Bull Entomol Res. 2013 Jun;103(3):251-60. doi: 10.1017/S0007485312000648. Epub 
      2012 Nov 1.