PMID- 23114126
OWN - NLM
STAT- MEDLINE
DCOM- 20131205
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 20
IP  - 5
DP  - 2012 Oct
TI  - [Evaluation of fluorescence in situ hybridization value in detection of chronic 
      lymphocytic leukemia].
PG  - 1095-8
AB  - The aim of this study was to investigate the clinical value of fluorescence in 
      situ hybridization (FISH) in detecting the genomic aberration of chronic 
      lymphocytic leukemia (CLL). FISH was used for 32 patients who were newly 
      diagnosed as CLL. Five types of fluorescence probes with labeled DNA probes were 
      included as sequence specific probes D13S25 for 13q14.3, P53 for 17p13.1, ATM for 
      11q22.3, RB1 for 13q14 and chromosomes 12. Meanwhile, FISH was used to detect 
      IGH/CCND1 fusion gene in 10 CLL patients with untypical immunophenotypes. The 
      results showed that out of 32 patients, 26 cases (81.3%) were abnormal including 
      14 cases of D13S25 deletion, 11 of RB1 deletion, 9 of trisomia 12, 6 cases of P53 
      deletion, and 1 of ATM deletion. 12 cases showed 1 kind of genomic aberration, 
      including 7 cases of trisomia 12, 3 cases of D13S25 deletion, 1 of P53 deletion, 
      1 of ATM deletion. 11 eases displayed 2 kinds of abnormalities. Out of 11 cases, 
      7 were D13S25/RB1 deletion, 4 were of P53 deletion, and 3 cases had 3 kinds of 
      abnormalities. Among 10 patients with CD5(+)CD23(-), two were positive with 
      IGH/CCND1. It is concluded that the FISH can improve the detecting of chromosomal 
      abnormalities in CLL, and every abnormality has its special feature. Detection of 
      IGH/CCND1 seems important in diagnoses of CLL.
FAU - Wu, Wei
AU  - Wu W
AD  - Yangzhou Municipal Institute of Hematology, Yangzhou, Jiangsu Province, China.
FAU - Gu, Jian
AU  - Gu J
FAU - Ma, Li
AU  - Ma L
FAU - Wang, Hong
AU  - Wang H
FAU - Ni, Jun
AU  - Ni J
FAU - Ji, Wei
AU  - Ji W
FAU - Shen, Lian-Jun
AU  - Shen LJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Sequence Deletion
EDAT- 2012/11/02 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 1009-2137(2012)05-1095-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Oct;20(5):1095-8.