PMID- 23114127
OWN - NLM
STAT- MEDLINE
DCOM- 20131205
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 20
IP  - 5
DP  - 2012 Oct
TI  - [Diagnostic value of fluorescence in situ hybridization for children with acute 
      myeloid leukemia].
PG  - 1099-102
AB  - This study was purposed to investigate the diagnostic value of fluorescence in 
      situ hybridization (FISH) technique for the childhood acute myeloid leukemia 
      (AML). The medical data of 179 children with AML (aged </= 16 years) were 
      retrospectively studied, who were initially diagnosed in our hospital from April 
      2005 to April 2010. Through the analysis of the results of FISH, chromosome 
      banding analysis and polymerase chain reaction, the difference and 
      complementarity between FISH and other 2 methods for detecting the fusion genes 
      were explored. The results indicated that the detection rate of genetic 
      abnormality with FISH was higher. The PML/RARalpha probe was used in 27 AML-M3 
      patients, 22 out of whom were evaluated as PML/RARalpha positive. The AML1/ETO probe 
      was used in 24 AML-M2b patients and all of them were evaluated as AML1/ETO 
      positive. The CBFbeta/MYH11 probe was used in 4 AML-M4Eo patients and all of them 
      were evaluated as CBFbeta/MYH11 positive. It is concluded that FISH is a sensitive 
      method for detecting fusion genes and the results of FISH have a good correlation 
      with the chromosome banding analysis and polymerase chain reaction. The 
      combination of FISH with other 2 methods improves the detection rate of genetic 
      abnormality, which is useful for the diagnosis and typing of childhood AML.
FAU - Wang, Ya-Qin
AU  - Wang YQ
AD  - Department of Pediatrics, Chinese Academy of Medical Sciences, Tianjin, China.
FAU - Zhou, Jian-Feng
AU  - Zhou JF
FAU - Ruan, Min
AU  - Ruan M
FAU - Yi, Xiao-Li
AU  - Yi XL
FAU - An, Wen-Bin
AU  - An WB
FAU - Yang, Wen-Yu
AU  - Yang WY
FAU - Guo, Ye
AU  - Guo Y
FAU - Zhang, Li
AU  - Zhang L
FAU - Liu, Tian-Feng
AU  - Liu TF
FAU - Zhu, Xiao-Fan
AU  - Zhu XF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*diagnosis/genetics
MH  - Male
MH  - Retrospective Studies
EDAT- 2012/11/02 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 1009-2137(2012)05-1099-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Oct;20(5):1099-102.