PMID- 23114406
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20211021
IS  - 1437-9813 (Electronic)
IS  - 0179-0358 (Linking)
VI  - 29
IP  - 1
DP  - 2013 Jan
TI  - Dietary supplementation with vitamin D stimulates intestinal epithelial cell 
      turnover after massive small bowel resection in rats.
PG  - 41-50
LID - 10.1007/s00383-012-3205-4 [doi]
AB  - PURPOSE: While the endocrine action of the active metabolite 
      1,25-dihydroxyvitamin D (VtD) has been well characterized in relation to the 
      maintenance of plasma calcium and phosphate homeostasis through regulation of 
      intestinal absorption, recent research has focused on its autocrine and/or 
      paracrine activities. Such activities have been best characterized in intestine, 
      where VtD regulates cell differentiation and maturation. The purpose of this 
      study was to evaluate the effect of VtD on enterocyte turnover in a rat model of 
      short bowel syndrome (SBS). METHODS: Male rats were divided into four groups: 
      sham rats underwent bowel transection, sham-VtD rats underwent bowel transection 
      and were treated oral VtD, SBS rats underwent a 75 % bowel resection, and SBS-VtD 
      rats underwent bowel resection and were treated with VtD. Parameters of 
      intestinal adaptation, enterocyte proliferation and apoptosis were determined at 
      sacrifice. Illumina's digital gene expression (DGE) analysis was used to 
      determine VtD pathway-related gene expression profiling. VtD receptor (VDR) and 
      its promoter, Bax and Bcl-2 mRNA expression were determined using real-time PCR. 
      Western blotting was used to determine p-ERK, Bax and beta-catenin protein levels. 
      RESULTS: From the total of 20,000 probes, 11 genes related to VtD signaling were 
      investigated. Of these genes, five were found to be up-regulated in SBS versus 
      sham animals with a relative change in gene expression level of 20 %, five 
      remained unchanged, and one was down-regulated. VtD treatment in sham and SBS 
      rats resulted in significant up-regulation of the VDR gene and its promoter's 
      expression. SBS-VtD rats demonstrated a significant increase in all intestinal 
      mucosal parameters compared to SBS animals. A significant increase in cell 
      proliferation in SBS-VtD rats was accompanied by increased beta-catenin protein 
      levels. A significant decrease in cell apoptosis in this group was correlated 
      with lower Bax/Bcl-2 mRNA and protein levels. CONCLUSION: In a rat model of SBS, 
      dietary supplementation with VtD stimulates enterocyte turnover, which correlates 
      with up-regulated VtD receptor expression in the remaining small intestine.
FAU - Hadjittofi, Christopher
AU  - Hadjittofi C
AD  - Laboratory of Intestinal Adaptation and Recovery, The Bruce Rappaport Faculty of 
      Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
FAU - Coran, Arnold G
AU  - Coran AG
FAU - Mogilner, Jorge G
AU  - Mogilner JG
FAU - Pollak, Yulia
AU  - Pollak Y
FAU - Matter, Ibrahim
AU  - Matter I
FAU - Sukhotnik, Igor
AU  - Sukhotnik I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pediatr Surg Int
JT  - Pediatric surgery international
JID - 8609169
RN  - 1406-16-2 (Vitamin D)
RN  - 66772-14-3 (1,25-dihydroxyvitamin D)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - *Dietary Supplements
MH  - Epithelial Cells/*cytology/*drug effects
MH  - Intestinal Mucosa/*cytology/*drug effects
MH  - Intestine, Small/*cytology/*surgery
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vitamin D/*analogs & derivatives/therapeutic use
EDAT- 2012/11/02 06:00
MHDA- 2013/06/26 06:00
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - 10.1007/s00383-012-3205-4 [doi]
PST - ppublish
SO  - Pediatr Surg Int. 2013 Jan;29(1):41-50. doi: 10.1007/s00383-012-3205-4.