PMID- 23114625
OWN - NLM
STAT- MEDLINE
DCOM- 20130917
LR  - 20211021
IS  - 1435-702X (Electronic)
IS  - 0721-832X (Linking)
VI  - 251
IP  - 5
DP  - 2013 May
TI  - In-vivo investigation of laser-induced choroidal neovascularization in rat using 
      spectral-domain optical coherence tomography (SD-OCT).
PG  - 1293-301
LID - 10.1007/s00417-012-2185-3 [doi]
AB  - PURPOSE: This study investigated the in-vivo formation process of laser-induced 
      choroidal neovascularization (CNV) in rat using high-resolution spectral-domain 
      optical coherence tomography (SD-OCT), and compared the results to histological 
      methods. METHODS: Brown Norway rats (n = 60, 6-8 weeks of age) received 532-nm 
      diode laser photocoagulation. SD-OCT and fluorescein angiography (FA) were 
      performed in vivo 2, 5, 7, 14, and 21 days post-laser application. Haematoxylin 
      and eosin (H&E) staining and immunohistochemistry for CD31, phosphorylated 
      vascular endothelial factor receptor 2 (pVEGFR2) were conducted at each time 
      point to observe the CNV in vitro. Choroidal flatmount preparations were observed 
      using a confocal laser scanning microscope (CLSM) and a scanning electron 
      microscope (SEM). RESULTS: SD-OCT monitored the longitudinal morphological 
      changes of laser-induced CNV. CNV reached its maximal size on day 7, and began a 
      gradual reduction on day 14. FA revealed similar dynamic changes in leakage. CNV 
      thickness, as assessed by SD-OCT, was consistent with H&E-stained sections at 
      each time point. CLSM and SEM revealed the details of the fibrovascular membrane. 
      CD31 and pVEGFR2 expression supported the results of SD-OCT and histology. 
      CONCLUSIONS: SD-OCT was a convenient and reliable tool for the imaging of the CNV 
      formation process and quantification of the lesion size in vivo.
FAU - Liu, Tao
AU  - Liu T
AD  - Department of Ophthalmology, Eye Institute of Chinese PLA, Xijing Hospital, 
      Fourth Military Medical University, Xi'an, 710032, China.
FAU - Hui, Ling
AU  - Hui L
FAU - Wang, Yu-sheng
AU  - Wang YS
FAU - Guo, Jian-qiang
AU  - Guo JQ
FAU - Li, Rong
AU  - Li R
FAU - Su, Jing-bo
AU  - Su JB
FAU - Chen, Jian-kang
AU  - Chen JK
FAU - Xin, Xue-mei
AU  - Xin XM
FAU - Li, Wei-hua
AU  - Li WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121101
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Animals
MH  - Choroid/ultrastructure
MH  - Choroidal Neovascularization/etiology/metabolism/*pathology
MH  - *Disease Models, Animal
MH  - Fluorescein Angiography
MH  - *Laser Coagulation
MH  - *Lasers, Semiconductor
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - *Tomography, Optical Coherence
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
EDAT- 2012/11/02 06:00
MHDA- 2013/09/18 06:00
CRDT- 2012/11/02 06:00
PHST- 2012/04/16 00:00 [received]
PHST- 2012/10/15 00:00 [accepted]
PHST- 2012/09/06 00:00 [revised]
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/09/18 06:00 [medline]
AID - 10.1007/s00417-012-2185-3 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2013 May;251(5):1293-301. doi: 
      10.1007/s00417-012-2185-3. Epub 2012 Nov 1.