PMID- 23115065
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20211021
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Print)
IS  - 1552-4973 (Linking)
VI  - 101
IP  - 2
DP  - 2013 Feb
TI  - Tailoring of processing parameters for sintering microsphere-based scaffolds with 
      dense-phase carbon dioxide.
PG  - 330-7
LID - 10.1002/jbm.b.32843 [doi]
AB  - Microsphere-based polymeric tissue-engineered scaffolds offer the advantage of 
      shape-specific constructs with excellent spatiotemporal control and 
      interconnected porous structures. The use of these highly versatile scaffolds 
      requires a method to sinter the discrete microspheres together into a cohesive 
      network, typically with the use of heat or organic solvents. We previously 
      introduced subcritical CO(2) as a sintering method for microsphere-based 
      scaffolds; here we further explored the effect of processing parameters. Gaseous 
      or subcritical CO(2) was used for making the scaffolds, and various pressures, 
      ratios of lactic acid to glycolic acid in poly(lactic acid-co-glycolic acid), and 
      amounts of NaCl particles were explored. By changing these parameters, scaffolds 
      with different mechanical properties and morphologies were prepared. The 
      preferred range of applied subcritical CO(2) was 15-25 bar. Scaffolds prepared at 
      25 bar with lower lactic acid ratios and without NaCl particles had a higher 
      stiffness, while the constructs made at 15 bar, lower glycolic acid content, and 
      with salt granules had lower elastic moduli. Human umbilical cord mesenchymal 
      stromal cells (hUCMSCs) seeded on the scaffolds demonstrated that cells penetrate 
      the scaffolds and remain viable. Overall, the study demonstrated the dependence 
      of the optimal CO(2) sintering parameters on the polymer and conditions, and 
      identified desirable CO(2) processing parameters to employ in the sintering of 
      microsphere-based scaffolds as a more benign alternative to heat-sintering or 
      solvent-based sintering methods.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Jeon, Ju Hyeong
AU  - Jeon JH
AD  - Integrated Department of Orthopaedics and Rehabilitation, Walter Reed AMC, 
      Washington, DC, USA.
FAU - Bhamidipati, Manjari
AU  - Bhamidipati M
FAU - Sridharan, BanuPriya
AU  - Sridharan B
FAU - Scurto, Aaron M
AU  - Scurto AM
FAU - Berkland, Cory J
AU  - Berkland CJ
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
GR  - R01 AR056347/AR/NIAMS NIH HHS/United States
GR  - R21 EB007313/EB/NIBIB NIH HHS/United States
PT  - Journal Article
DEP - 20121031
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 142M471B3J (Carbon Dioxide)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Biomechanical Phenomena
MH  - Carbon Dioxide
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Humans
MH  - Lactic Acid
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/cytology
MH  - Microscopy, Electron, Scanning
MH  - Microspheres
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Porosity
MH  - Tissue Engineering/instrumentation/methods
MH  - *Tissue Scaffolds
MH  - Umbilical Cord/cytology
PMC - PMC4474405
MID - NIHMS699668
EDAT- 2012/11/02 06:00
MHDA- 2013/06/26 06:00
PMCR- 2015/06/19
CRDT- 2012/11/02 06:00
PHST- 2012/02/06 00:00 [received]
PHST- 2012/08/01 00:00 [revised]
PHST- 2012/09/11 00:00 [accepted]
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
PHST- 2015/06/19 00:00 [pmc-release]
AID - 10.1002/jbm.b.32843 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2013 Feb;101(2):330-7. doi: 
      10.1002/jbm.b.32843. Epub 2012 Oct 31.