PMID- 23115247
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 53
DP  - 2012 Dec 28
TI  - Functional glycomic analysis of human milk glycans reveals the presence of virus 
      receptors and embryonic stem cell biomarkers.
PG  - 44784-99
LID - 10.1074/jbc.M112.425819 [doi]
AB  - Human milk contains a large diversity of free glycans beyond lactose, but their 
      functions are not well understood. To explore their functional recognition, here 
      we describe a shotgun glycan microarray prepared from isolated human milk glycans 
      (HMGs), and our studies on their recognition by viruses, antibodies, and 
      glycan-binding proteins (GBPs), including lectins. The total neutral and 
      sialylated HMGs were derivatized with a bifunctional fluorescent tag, separated 
      by multidimensional HPLC, and archived in a tagged glycan library, which was then 
      used to print a shotgun glycan microarray (SGM). This SGM was first interrogated 
      with well defined GBPs and antibodies. These data demonstrated both the utility 
      of the array and provided preliminary structural information (metadata) about 
      this complex glycome. Anti-TRA-1 antibodies that recognize human pluripotent stem 
      cells specifically recognized several HMGs that were then further structurally 
      defined as novel epitopes for these antibodies. Human influenza viruses and 
      Parvovirus Minute Viruses of Mice also specifically recognized several HMGs. For 
      glycan sequencing, we used a novel approach termed metadata-assisted glycan 
      sequencing (MAGS), in which we combine information from analyses of glycans by 
      mass spectrometry with glycan interactions with defined GBPs and antibodies 
      before and after exoglycosidase treatments on the microarray. Together, these 
      results provide novel insights into diverse recognition functions of HMGs and 
      show the utility of the SGM approach and MAGS as resources for defining novel 
      glycan recognition by GBPs, antibodies, and pathogens.
FAU - Yu, Ying
AU  - Yu Y
AD  - Department of Biochemistry and the Glycomics Center, Emory University School of 
      Medicine, Atlanta, Georgia 30322, USA.
FAU - Mishra, Shreya
AU  - Mishra S
FAU - Song, Xuezheng
AU  - Song X
FAU - Lasanajak, Yi
AU  - Lasanajak Y
FAU - Bradley, Konrad C
AU  - Bradley KC
FAU - Tappert, Mary M
AU  - Tappert MM
FAU - Air, Gillian M
AU  - Air GM
FAU - Steinhauer, David A
AU  - Steinhauer DA
FAU - Halder, Sujata
AU  - Halder S
FAU - Cotmore, Susan
AU  - Cotmore S
FAU - Tattersall, Peter
AU  - Tattersall P
FAU - Agbandje-McKenna, Mavis
AU  - Agbandje-McKenna M
FAU - Cummings, Richard D
AU  - Cummings RD
FAU - Smith, David F
AU  - Smith DF
LA  - eng
GR  - HHSN266200700006C/AI/NIAID NIH HHS/United States
GR  - P41 GM103694/GM/NIGMS NIH HHS/United States
GR  - R01 GM085448/GM/NIGMS NIH HHS/United States
GR  - GM62116/GM/NIGMS NIH HHS/United States
GR  - U54 GM062116/GM/NIGMS NIH HHS/United States
GR  - R01 CA029303/CA/NCI NIH HHS/United States
GR  - R24 GM098791/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121031
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Biomarkers)
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, Virus)
SB  - IM
MH  - Animals
MH  - Biomarkers/*chemistry
MH  - Carbohydrate Sequence
MH  - Cell Line
MH  - Embryonic Stem Cells/metabolism
MH  - *Glycomics
MH  - Humans
MH  - Mice
MH  - Milk, Human/*chemistry/metabolism
MH  - Molecular Sequence Data
MH  - Polysaccharides/*chemistry/metabolism
MH  - Receptors, Virus/*analysis/genetics/metabolism
PMC - PMC3531791
EDAT- 2012/11/02 06:00
MHDA- 2013/03/26 06:00
PMCR- 2013/12/28
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
PHST- 2013/12/28 00:00 [pmc-release]
AID - S0021-9258(20)41713-2 [pii]
AID - M112.425819 [pii]
AID - 10.1074/jbc.M112.425819 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Dec 28;287(53):44784-99. doi: 10.1074/jbc.M112.425819. Epub 
      2012 Oct 31.