PMID- 23115280
OWN - NLM
STAT- MEDLINE
DCOM- 20130225
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 87
IP  - 2
DP  - 2013 Jan
TI  - A second CRM1-dependent nuclear export signal in the influenza A virus NS2 
      protein contributes to the nuclear export of viral ribonucleoproteins.
PG  - 767-78
LID - 10.1128/JVI.06519-11 [doi]
AB  - Influenza A virus NS2 protein, also called nuclear export protein (NEP), is 
      crucial for the nuclear export of viral ribonucleoproteins. However, the 
      molecular mechanisms of NEP mediation in this process remain incompletely 
      understood. A leucine-rich nuclear export signal (NES2) in NEP, located at the 
      predicted N2 helix of the N-terminal domain, was identified in the present study. 
      NES2 was demonstrated to be a transferable NES, with its nuclear export activity 
      depending on the nuclear export receptor chromosome region maintenance 1 
      (CRM1)-mediated pathway. The interaction between NEP and CRM1 is coordinately 
      regulated by both the previously reported NES (NES1) and now the new NES2. 
      Deletion of the NES1 enhances the interaction between NEP and CRM1, and deletion 
      of the NES1 and NES2 motifs completely abolishes this interaction. Moreover, NES2 
      interacts with CRM1 in the mammalian two-hybrid system. Mutant viruses containing 
      NES2 alterations generated by reversed genetics exhibit reduced viral growth and 
      delay in the nuclear export of viral ribonucleoproteins (vRNPs). The NES2 motif 
      is highly conserved in the influenza A and B viruses. The results demonstrate 
      that leucine-rich NES2 is involved in the nuclear export of vRNPs and contributes 
      to the understanding of nucleocytoplasmic transport of influenza virus vRNPs.
FAU - Huang, Shengping
AU  - Huang S
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan, China.
FAU - Chen, Jingjing
AU  - Chen J
FAU - Chen, Quanjiao
AU  - Chen Q
FAU - Wang, Huadong
AU  - Wang H
FAU - Yao, Yanfeng
AU  - Yao Y
FAU - Chen, Jianjun
AU  - Chen J
FAU - Chen, Ze
AU  - Chen Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121031
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (NS2 protein, influenza virus A)
RN  - 0 (Nuclear Export Signals)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - *Active Transport, Cell Nucleus
MH  - Cell Line
MH  - Conserved Sequence
MH  - Humans
MH  - Influenza A virus/genetics/*physiology
MH  - *Nuclear Export Signals
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Ribonucleoproteins/*metabolism
MH  - Sequence Deletion
MH  - Two-Hybrid System Techniques
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - *Virus Replication
PMC - PMC3554077
EDAT- 2012/11/02 06:00
MHDA- 2013/02/26 06:00
PMCR- 2013/07/01
CRDT- 2012/11/02 06:00
PHST- 2012/11/02 06:00 [entrez]
PHST- 2012/11/02 06:00 [pubmed]
PHST- 2013/02/26 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - JVI.06519-11 [pii]
AID - 06519-11 [pii]
AID - 10.1128/JVI.06519-11 [doi]
PST - ppublish
SO  - J Virol. 2013 Jan;87(2):767-78. doi: 10.1128/JVI.06519-11. Epub 2012 Oct 31.