PMID- 2311582
OWN - NLM
STAT- MEDLINE
DCOM- 19900420
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 9
IP  - 3
DP  - 1990 Mar
TI  - Domains involved in multimer assembly of von willebrand factor (vWF): 
      multimerization is independent of dimerization.
PG  - 797-803
AB  - The precursor protein of von Willebrand factor (pro-vWF) consist of four repeated 
      domains, denoted D1-D2-D'-D3-A1-A2-A3-D4-B1-B2-B3-C1-C2. The domains D1 and D2 
      constitute the amino-terminal pro-polypeptide and the remaining domains mature 
      vWF, generated upon proteolytic processing. We have shown previously that the 
      pro-polypeptide of pro-vWF is obligatory for assembly of pro-vWF dimers into 
      multimers, a process vital for efficient adhesion of platelets to an injured 
      vessel wall. Here, we have employed full length vWF cDNA to construct a series of 
      deletion mutants, based on the homology between the various domains. 
      Specifically, the domains D', D3 or both were deleted and the multimeric pattern 
      of the mutant vWF proteins was analysed after transient expression in COS-1 
      cells. It is demonstrated that in addition to the pro-polypeptide, both the D' 
      and the D3 domain are required for multimer assembly. Furthermore, by analysing a 
      construct containing only the domains D' and D3 next to the pro-polypeptide it is 
      shown that this is the only part of the vWF protein involved in multimer 
      assembly. Since, the formation of pro-vWF dimers relies on the carboxy-terminal 
      area of mature vWF, it is concluded that multimer assembly is a process 
      independent of dimerization.
FAU - Voorberg, J
AU  - Voorberg J
AD  - Department of Molecular Biology, the Netherlands Red Cross Blood Transfusion 
      Service, Amsterdam.
FAU - Fontijn, R
AU  - Fontijn R
FAU - van Mourik, J A
AU  - van Mourik JA
FAU - Pannekoek, H
AU  - Pannekoek H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Macromolecular Substances)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (von Willebrand Factor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosome Deletion
MH  - Gene Expression
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Oligonucleotide Probes
MH  - Plasmids
MH  - *Protein Processing, Post-Translational
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - Transfection
MH  - von Willebrand Factor/*genetics
PMC - PMC551739
EDAT- 1990/03/01 00:00
MHDA- 1990/03/01 00:01
PMCR- 1991/03/01
CRDT- 1990/03/01 00:00
PHST- 1990/03/01 00:00 [pubmed]
PHST- 1990/03/01 00:01 [medline]
PHST- 1990/03/01 00:00 [entrez]
PHST- 1991/03/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1990.tb08176.x [doi]
PST - ppublish
SO  - EMBO J. 1990 Mar;9(3):797-803. doi: 10.1002/j.1460-2075.1990.tb08176.x.