PMID- 23118515
OWN - NLM
STAT- MEDLINE
DCOM- 20130411
LR  - 20230502
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2012
DP  - 2012
TI  - Analysis of casein biopolymers adsorption to lignocellulosic biomass as a 
      potential cellulase stabilizer.
PG  - 745181
LID - 10.1155/2012/745181 [doi]
LID - 745181
AB  - Although lignocellulosic materials have a good potential to substitute current 
      feedstocks used for ethanol production, conversion of these materials to 
      fermentable sugars is still not economical through enzymatic hydrolysis. High 
      cost of cellulase has prompted research to explore techniques that can prevent 
      from enzyme deactivation. Colloidal proteins of casein can form monolayers on 
      hydrophobic surfaces that alleviate the de-activation of protein of interest. 
      Scanning electron microscope (SEM), fourier transform infrared spectroscopy 
      (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were 
      used to investigate the unknown mechanism of action of induced cellulase activity 
      during hydrolysis of casein-treated biomass. Adsorption of casein to biomass was 
      observed with all of the analytical techniques used and varied depending on the 
      pretreatment techniques of biomass. FT-IR analysis of amides I and II suggested 
      that the substructure of protein from casein or skim milk were deformed at the 
      time of contact with biomass. With no additive, the majority of one of the 
      cellulase mono-component, 97.1 +/- 1.1, was adsorbed to CS within 24 h, this 
      adsorption was irreversible and increased by 2% after 72 h. However, biomass 
      treatment with skim-milk and casein reduced the adsorption to 32.9% +/- 6.0 and 
      82.8% +/- 6.0, respectively.
FAU - Eckard, Anahita Dehkhoda
AU  - Eckard AD
AD  - Department of Agricultural and Biosystems Engineering, South Dakota State 
      University, Brookings, SD 57007, USA. anahita.eckard@sdstate.edu
FAU - Muthukumarappan, Kasiviswanathan
AU  - Muthukumarappan K
FAU - Gibbons, William
AU  - Gibbons W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121014
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
RN  - 0 (Alkalies)
RN  - 0 (Biopolymers)
RN  - 0 (Caseins)
RN  - 0 (Milk Proteins)
RN  - 0 (Solutions)
RN  - 0 (Whey Proteins)
RN  - 11132-73-3 (lignocellulose)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 9005-53-2 (Lignin)
RN  - EC 3.2.1.4 (Cellulase)
SB  - IM
MH  - Adsorption/drug effects
MH  - Alkalies/pharmacology
MH  - Animals
MH  - *Biomass
MH  - Biopolymers/*metabolism
MH  - Caseins/*metabolism/ultrastructure
MH  - Cattle
MH  - Cellulase/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Electrophoresis, Capillary
MH  - Enzyme Stability/drug effects
MH  - Hydrolysis/drug effects
MH  - Lignin/*metabolism
MH  - Milk Proteins/metabolism
MH  - Serum Albumin, Bovine/metabolism
MH  - Solubility/drug effects
MH  - Solutions
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Whey Proteins
MH  - Zea mays/chemistry
PMC - PMC3481958
EDAT- 2012/11/03 06:00
MHDA- 2013/04/12 06:00
PMCR- 2012/10/14
CRDT- 2012/11/03 06:00
PHST- 2012/05/23 00:00 [received]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2012/11/03 06:00 [entrez]
PHST- 2012/11/03 06:00 [pubmed]
PHST- 2013/04/12 06:00 [medline]
PHST- 2012/10/14 00:00 [pmc-release]
AID - 10.1155/2012/745181 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2012;2012:745181. doi: 10.1155/2012/745181. Epub 2012 Oct 
      14.