PMID- 23119140
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121105
LR  - 20211021
IS  - 2041-5346 (Print)
IS  - 2041-5346 (Electronic)
IS  - 2041-5346 (Linking)
VI  - 17
IP  - 1
DP  - 2010
TI  - Activation of kinase phosphorylation by heat-shift and mild heat-shock.
PG  - e00002
LID - 10.1042/CBR20100002 [doi]
LID - e00002
AB  - Most cells activate intracellular signalling to recover from heat damage. An 
      increase of temperature, known as HS (heat shock), induces two major signalling 
      events: the transcriptional induction of HSPs (heat-shock proteins) and the 
      activation of the MAPK (mitogen-activated protein kinase) cascade. We performed 
      the present study to examine the effects of HS, induced by different experimental 
      conditions, on various kinases [ERK (extracellular-signal-regulated kinase), JNK 
      (c-Jun N-terminal kinase), p38, Akt, AMPK (AMP-activated protein kinase) and PKC 
      (protein kinase C)]. We investigated by Western blot analysis the phosphorylation 
      of MAPK as a measure of cellular responsiveness to heat shift (37 degrees C) and mild HS 
      (40 degrees C) in different cell lines. The results of the study indicate that every cell 
      line responded to heat shift, and to a greater extent to HS, increasing ERK and 
      JNK phosphorylation, whereas variable effects on activation or inhibition of PKC, 
      AMPK, Akt and p38 were observed. Besides the implications of intracellular 
      signalling activated by heat variations, these data may be of technical 
      relevance, indicating possible sources of error due to different experimental 
      temperature conditions.
FAU - Petrocchi, Pamela
AU  - Petrocchi P
AD  - Institute of Neurobiology and Molecular Medicine, CNR, Via del Fosso di Fiorano 
      65, 00143 Rome, Italy.
FAU - Quaresima, Stefania
AU  - Quaresima S
FAU - Mongiardi, Maria Patrizia
AU  - Mongiardi MP
FAU - Severini, Cinzia
AU  - Severini C
FAU - Possenti, Roberta
AU  - Possenti R
LA  - eng
PT  - Journal Article
DEP - 20100803
PL  - England
TA  - Cell Biol Int Rep (2010)
JT  - Cell biology international reports
JID - 101574535
PMC - PMC3476822
OTO - NOTNLM
OT  - AMPK, AMP-activated protein kinase
OT  - CGC, cerebellar granule cell
OT  - CHO, Chinese-hamster ovary
OT  - ERK, extracellular-signal-regulated kinase
OT  - HS, heat shock
OT  - JNK, c-Jun N-terminal kinase
OT  - MAPK, mitogen-activated protein kinase
OT  - PKC, protein kinase C
OT  - cell culture
OT  - heat damage
OT  - signal transduction
EDAT- 2010/01/01 00:00
MHDA- 2010/01/01 00:01
PMCR- 2010/08/03
CRDT- 2012/11/03 06:00
PHST- 2010/04/19 00:00 [received]
PHST- 2010/04/21 00:00 [accepted]
PHST- 2012/11/03 06:00 [entrez]
PHST- 2010/01/01 00:00 [pubmed]
PHST- 2010/01/01 00:01 [medline]
PHST- 2010/08/03 00:00 [pmc-release]
AID - e00002 [pii]
AID - 10.1042/CBR20100002 [doi]
PST - ppublish
SO  - Cell Biol Int Rep (2010). 2010;17(1):e00002. doi: 10.1042/CBR20100002. Epub 2010 
      Aug 3.