PMID- 23121457
OWN - NLM
STAT- MEDLINE
DCOM- 20140619
LR  - 20161125
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 84
IP  - 9
DP  - 2013 Sep
TI  - Histometric analysis of the effect of enamel matrix derivative on the healing of 
      periodontal defects in rats with diabetes.
PG  - 1309-18
LID - 10.1902/jop.2012.120354 [doi]
AB  - BACKGROUND: Diabetes mellitus (DM) involves metabolic changes that can negatively 
      influence periodontal tissues, resulting in impaired periodontal repair. There is 
      a lack of information about the outcomes of regenerative approaches under the 
      influence of DM. Enamel matrix derivatives (EMDs) have been used in periodontal 
      regenerative procedures, resulting in improvement of clinical parameters. Thus, 
      the aim of this histomorphometric study is to evaluate the healing of periodontal 
      defects after treatment with EMD under the influence of DM. METHODS: Twenty 
      Wistar rats were randomly assigned to two groups: group 1 (G1): DM was induced 
      with a single intraperitoneal injection of streptozotocin (STZ) (n = 10); group 2 
      (G2): rats were not exposed to STZ (n = 10). Seven days after DM induction, 
      bilateral fenestration defects were created at the buccal aspect of the first 
      mandibular molar. After the surgeries, the defects of each animal were randomly 
      assigned to two subgroups: non-treated (control) and treated with EMD. The 
      animals were euthanized 21 days later, and the percentage of defect fill (DF), 
      newly formed bone density (BD), and new cementum formation (NCF) were 
      histometrically assessed. The number of osteoclasts was determined by 
      tartrate-resistant acid phosphatase. Weight and blood glucose were also analyzed. 
      Mann-Whitney U test was used for comparison among groups and Wilcoxon test for 
      comparison between the start and end times (weight and blood glucose) and between 
      treatments (NCF and number of osteoclasts). One-way analysis of variance was used 
      to assess DF and BD. Tukey test was used when the analysis of variance test 
      detected significant differences (alpha = 5%). RESULTS: G1 (DM) showed less DF and BD 
      compared with G2. EMD provided an increased DF in both groups and enhanced BD and 
      NCF only in G2. The number of TRAP-positive osteoclasts was significantly higher 
      in EMD-treated sites of G1. CONCLUSIONS: DM may produce a significant detrimental 
      effect on BD. EMD may provide greater DF under diabetic or normal conditions; 
      however, it may not significantly increase NCF in animals with DM.
FAU - Correa, Monica Grazieli
AU  - Correa MG
AD  - Department of Prosthodontics and Periodontics, Division of Periodontics, 
      Piracicaba Dental School, University of Campinas, Piracicaba, Sao Paulo, Brazil.
FAU - Gomes Campos, Mirella L
AU  - Gomes Campos ML
FAU - Marques, Marcelo R
AU  - Marques MR
FAU - Casati, Marcio Z
AU  - Casati MZ
FAU - Nociti, Francisco H Jr
AU  - Nociti FH Jr
FAU - Sallum, Enilson A
AU  - Sallum EA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121103
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Blood Glucose)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Placebos)
RN  - 0 (enamel matrix proteins)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/analysis
MH  - Alveolar Bone Loss/pathology/*surgery
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Body Weight
MH  - Bone Density/drug effects
MH  - Cementogenesis/drug effects
MH  - Dental Cementum/pathology
MH  - Dental Enamel Proteins/*therapeutic use
MH  - Diabetes Mellitus, Experimental/blood/*physiopathology
MH  - Guided Tissue Regeneration, Periodontal/methods
MH  - Isoenzymes/analysis
MH  - Male
MH  - Osteoclasts/pathology
MH  - Osteogenesis/drug effects
MH  - Placebos
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Streptozocin
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Time Factors
MH  - Treatment Outcome
MH  - Wound Healing/physiology
EDAT- 2012/11/06 06:00
MHDA- 2014/06/20 06:00
CRDT- 2012/11/06 06:00
PHST- 2012/11/06 06:00 [entrez]
PHST- 2012/11/06 06:00 [pubmed]
PHST- 2014/06/20 06:00 [medline]
AID - 10.1902/jop.2012.120354 [doi]
PST - ppublish
SO  - J Periodontol. 2013 Sep;84(9):1309-18. doi: 10.1902/jop.2012.120354. Epub 2012 
      Nov 3.