PMID- 23121623
OWN - NLM
STAT- MEDLINE
DCOM- 20130719
LR  - 20181202
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 56
IP  - 2
DP  - 2013 Feb
TI  - Analysis of microbial diversity on deli slicers using polymerase chain reaction 
      and denaturing gradient gel electrophoresis technologies.
PG  - 111-9
LID - 10.1111/lam.12021 [doi]
AB  - Cross-contamination of pathogenic and spoilage bacteria from food-contact 
      surfaces to food products is a serious public health issue. Bacteria may survive 
      and attach to food-contact surfaces by residual food components and/or background 
      bacteria which may subsequently transfer to other food products. Deli slicers, 
      generally used for slicing ready-to-eat products, can serve as potential sources 
      for considerable bacterial transfer. The objective of this study was to assess 
      the extent and distribution of microbial diversity of deli slicers by 
      identification of pathogenic and background bacteria. Slicer-swab samples were 
      collected from restaurants in Arkansas and Texas in the United States. Ten 
      surface areas for each slicer were swabbed using sterile sponges. Denaturing 
      gradient gel electrophoresis (DGGE) was applied to investigate the fingerprint of 
      samples, and each band was further identified by sequence analysis. Pseudomonads 
      were identified as the dominant bacteria followed by Enterobacteriaceae family, 
      and lactic acid bacteria such as Lactococcus lactis and Streptococcus 
      thermophilus were also found. Bacterial distribution was similar for all surface 
      areas, while the blade guard exhibited the greatest diversity. This study 
      provides a profile of the microbial ecology of slicers using DGGE to develop more 
      specific sanitation practices and to reduce cross-contamination during slicing.
CI  - (c) 2012 The Society for Applied Microbiology.
FAU - Koo, O K
AU  - Koo OK
AD  - Center for Food Safety, Food Science Department, University of Arkansas, 
      Fayetteville, AR, USA.
FAU - Mertz, A W
AU  - Mertz AW
FAU - Akins, E L
AU  - Akins EL
FAU - Sirsat, S A
AU  - Sirsat SA
FAU - Neal, J A
AU  - Neal JA
FAU - Morawicki, R
AU  - Morawicki R
FAU - Crandall, P G
AU  - Crandall PG
FAU - Ricke, S C
AU  - Ricke SC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121121
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacteria/classification/*isolation & purification
MH  - *Biodiversity
MH  - DNA, Bacterial/isolation & purification
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Food Contamination/prevention & control
MH  - Food Handling/*instrumentation
MH  - *Food Microbiology
MH  - Polymerase Chain Reaction
MH  - Restaurants
MH  - Sanitation
EDAT- 2012/11/06 06:00
MHDA- 2013/07/20 06:00
CRDT- 2012/11/06 06:00
PHST- 2012/06/15 00:00 [received]
PHST- 2012/10/10 00:00 [revised]
PHST- 2012/10/26 00:00 [accepted]
PHST- 2012/11/06 06:00 [entrez]
PHST- 2012/11/06 06:00 [pubmed]
PHST- 2013/07/20 06:00 [medline]
AID - 10.1111/lam.12021 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2013 Feb;56(2):111-9. doi: 10.1111/lam.12021. Epub 2012 Nov 
      21.