PMID- 23124633
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121106
LR  - 20211021
IS  - 2041-5346 (Electronic)
IS  - 2041-5346 (Linking)
VI  - 19
IP  - 2
DP  - 2012 Sep 6
TI  - hMSCs possess the potential to differentiate into DP cells in vivo and in vitro.
PG  - e00019
LID - 10.1042/CBR20120003 [doi]
LID - e00019
AB  - DP (dermal papilla) is a mesenchyme-derived structure situated at the base of the 
      HF (hair follicle) that plays an important role in embryonic hair morphogenesis 
      and maintenance of the hair growth cycle. hMSCs (human mesenchymal stem cells) 
      have gained widespread attention in the field of tissue engineering, but not much 
      is known about the differentiation of hMSCs into DP cells. hMSCs involved in HF 
      formation were examined in our previous study. Here, we have explored the 
      differentiation potential of hMSCs into DP cells by co-culturing hMSCs with DP 
      cells, which proved to be the case. During the differentiation process, the 
      expression of versican, CD133, SCF (stem cell factor), ET-1 (endothelin-1) and 
      bFGF (basic fibroblast growth factor) increased. Compared with hMSCs alone, the 
      aggregate number clearly increased when co-cultured with DP cells. The expression 
      in vivo of HLA-I (human leucocyte antigen class I) was confined to DP of the 
      newly formed HF. The data suggest that hMSCs possess the potential to 
      differentiate into DP cells in vivo and in vitro.
FAU - Wu, Minjuan
AU  - Wu M
AD  - Department of Histology and Embryology and Research Center of Developmental 
      Biology, Second Military Medical University, Shanghai, 200433, People's Republic 
      of China.
FAU - Sun, Qing
AU  - Sun Q
FAU - Guo, Xiaocan
AU  - Guo X
FAU - Liu, Houqi
AU  - Liu H
LA  - eng
PT  - Journal Article
DEP - 20120906
PL  - England
TA  - Cell Biol Int Rep (2010)
JT  - Cell biology international reports
JID - 101574535
PMC - PMC3475446
OTO - NOTNLM
OT  - DMEM, Dulbecco's minimum essential medium
OT  - DP, dermal papilla
OT  - ET-1, endothelin-1
OT  - FBS, fetal bovine serum
OT  - H&E, haematoxylin and eosin
OT  - HF, hair follicle
OT  - HLA-I, human leucocyte antigen class 1
OT  - RT-PCR, reverse transcription-PCR
OT  - SCF, stem cell factor
OT  - bFGF, basic fibroblast growth factor
OT  - co-culture
OT  - dermal papilla cells
OT  - differentiation
OT  - hMSC, human mesenchymal stem cell
OT  - hair follicle
OT  - human mesenchymal stem cells
OT  - alpha-SMA, alpha-smooth muscle actin
EDAT- 2012/11/06 06:00
MHDA- 2012/11/06 06:01
PMCR- 2012/09/06
CRDT- 2012/11/06 06:00
PHST- 2012/06/25 00:00 [received]
PHST- 2012/06/28 00:00 [accepted]
PHST- 2012/11/06 06:00 [entrez]
PHST- 2012/11/06 06:00 [pubmed]
PHST- 2012/11/06 06:01 [medline]
PHST- 2012/09/06 00:00 [pmc-release]
AID - e00019 [pii]
AID - 10.1042/CBR20120003 [doi]
PST - epublish
SO  - Cell Biol Int Rep (2010). 2012 Sep 6;19(2):e00019. doi: 10.1042/CBR20120003.