PMID- 23125915
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121106
LR  - 20211021
IS  - 2090-0015 (Electronic)
IS  - 2090-0007 (Print)
IS  - 2090-0007 (Linking)
VI  - 2012
DP  - 2012
TI  - Gene transfer of heme oxygenase-1 using an adeno-associated virus serotype 6 
      vector prolongs cardiac allograft survival.
PG  - 740653
LID - 10.1155/2012/740653 [doi]
LID - 740653
AB  - Introduction. Allograft survival can be prolonged by overexpression of 
      cytoprotective genes such as heme oxygenase-1 (HO-1). Modifications in vector 
      design and delivery have provided new opportunities to safely and effectively 
      administer HO-1 into the heart prior to transplantation to improve long-term 
      graft outcome. Methods. HO-1 was delivered to the donor heart using an 
      adeno-associated virus vector (AAV) with a pseudotype 6 capsid and vascular 
      endothelial growth factor (VEGF) to enhance myocardial tropism and microvascular 
      permeability. Survival of mouse cardiac allografts, fully or partially mismatched 
      at the MHC, was determined with and without cyclosporine A. Intragraft cytokine 
      gene expression was examined by PCR. Results. The use of AAV6 to deliver HO-1 to 
      the donor heart, combined with immunosuppression, prolonged allograft survival by 
      55.3% when donor and recipient were completely mismatched at the MHC and by 94.6% 
      if partially mismatched. The combination of gene therapy and immunosuppression 
      was more beneficial than treatment with either AAV6-HO-1 or CsA alone. IL-17a, b, 
      e and f were induced in the heart at rejection. Conclusions. Pretreatment of 
      cardiac allografts with AAV6-HO-1 plus cyclosporine A prolonged graft survival. 
      HO-1 gene therapy represents a beneficial adjunct to immunosuppressive therapy in 
      cardiac transplantation.
FAU - Evans, Jacqueline M
AU  - Evans JM
AD  - Department of Anesthesiology Critical Care Medicine, Children's Hospital Los 
      Angeles, Los Angeles, CA 90027, USA.
FAU - Navarro, Sonia
AU  - Navarro S
FAU - Doki, Tomoko
AU  - Doki T
FAU - Stewart, John M
AU  - Stewart JM
FAU - Mitsuhashi, Noboru
AU  - Mitsuhashi N
FAU - Kearns-Jonker, Mary
AU  - Kearns-Jonker M
LA  - eng
PT  - Journal Article
DEP - 20121016
PL  - United States
TA  - J Transplant
JT  - Journal of transplantation
JID - 101519118
PMC - PMC3480008
EDAT- 2012/11/06 06:00
MHDA- 2012/11/06 06:01
PMCR- 2012/10/16
CRDT- 2012/11/06 06:00
PHST- 2012/05/18 00:00 [received]
PHST- 2012/09/07 00:00 [revised]
PHST- 2012/09/07 00:00 [accepted]
PHST- 2012/11/06 06:00 [entrez]
PHST- 2012/11/06 06:00 [pubmed]
PHST- 2012/11/06 06:01 [medline]
PHST- 2012/10/16 00:00 [pmc-release]
AID - 10.1155/2012/740653 [doi]
PST - ppublish
SO  - J Transplant. 2012;2012:740653. doi: 10.1155/2012/740653. Epub 2012 Oct 16.