PMID- 23127173
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130118
LR  - 20211021
IS  - 2049-1891 (Electronic)
IS  - 1674-9782 (Print)
IS  - 1674-9782 (Linking)
VI  - 3
IP  - 1
DP  - 2012 Nov 6
TI  - Early-age feed restriction affects viability and gene expression of satellite 
      cells isolated from the gastrocnemius muscle of broiler chicks.
PG  - 33
LID - 10.1186/2049-1891-3-33 [doi]
AB  - BACKGROUND: Muscle growth depends on the fusion of proliferate satellite cells to 
      existing myofibers. We reported previously that 0-14 day intermittent feeding led 
      to persistent retardation in myofiber hypertrophy. However, how satellite cells 
      respond to such nutritional insult has not been adequately elucidated. RESULTS: 
      One-day-old broiler chicks were allocated to control (Con, ad libitum feeding), 
      intermittent feeding (IF, feed provided on alternate days) and re-feeding (RF, 2 
      days ad libitum feeding after 12 days of intermittent feeding) groups. Chickens 
      were killed on Day 15 and satellite cells were isolated. When cultured, satellite 
      cells from the IF group demonstrated significant retardation in proliferation and 
      differentiation potential, while RF partly restored the proliferation rate and 
      differentiation potential of the satellite cells. Significant up-regulation of 
      insulin like growth factor I receptor (IGF-IR) (P<0.05) and thyroid hormone 
      receptor alpha (TRalpha) (P<0.05), and down-regulation of growth hormone receptor (GHR) 
      (P<0.01) and IGF-I (P<0.01) mRNA expression was observed in freshly isolated IF 
      satellite cells when compared with Con cells. In RF cells, the mRNA expression of 
      IGF-I was higher (P<0.05) and of TRalpha was lower (P<0.01) than in IF cells, 
      suggesting that RF restored the mRNA expression of TRalpha and IGF-I, but not of GHR 
      and IGF-IR. The Bax/Bcl-2 ratio tended to increase in the IF group, which was 
      reversed in the RF group (P<0.05), indicating that RF reduced the pro-apoptotic 
      influence of IF. Moreover, no significant effect of T3 was detected on cell 
      survival in IF cells compared with Con (P<0.001) or RF (P<0.05) cells. 
      CONCLUSIONS: These data suggest that early-age feed restriction inhibits the 
      proliferation and differentiation of satellite cells, induces changes in mRNA 
      expression of the GH/IGF-I and thyroid hormone receptors in satellite cells, as 
      well as blunted sensitivity of satellite cells to T3, and that RF partially 
      reverses these effects. Thus, a moderate nutritional strategy for feed 
      restriction should be chosen in early chick rearing systems.
FAU - Li, Yue
AU  - Li Y
AD  - Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural 
      University, Nanjing 210095, China. zhao.ruqian@gmail.com.
FAU - Yang, Xiaojing
AU  - Yang X
FAU - Ni, Yingdong
AU  - Ni Y
FAU - Decuypere, Eddy
AU  - Decuypere E
FAU - Buyse, Johan
AU  - Buyse J
FAU - Everaert, Nadia
AU  - Everaert N
FAU - Grossmann, Roland
AU  - Grossmann R
FAU - Zhao, Ruqian
AU  - Zhao R
LA  - eng
PT  - Journal Article
DEP - 20121106
PL  - England
TA  - J Anim Sci Biotechnol
JT  - Journal of animal science and biotechnology
JID - 101581293
PMC - PMC3546929
EDAT- 2012/11/07 06:00
MHDA- 2012/11/07 06:01
PMCR- 2012/11/06
CRDT- 2012/11/07 06:00
PHST- 2012/05/15 00:00 [received]
PHST- 2012/11/01 00:00 [accepted]
PHST- 2012/11/07 06:00 [entrez]
PHST- 2012/11/07 06:00 [pubmed]
PHST- 2012/11/07 06:01 [medline]
PHST- 2012/11/06 00:00 [pmc-release]
AID - 2049-1891-3-33 [pii]
AID - 10.1186/2049-1891-3-33 [doi]
PST - epublish
SO  - J Anim Sci Biotechnol. 2012 Nov 6;3(1):33. doi: 10.1186/2049-1891-3-33.