PMID- 23128095
OWN - NLM
STAT- MEDLINE
DCOM- 20141030
LR  - 20181203
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 23
IP  - 1
DP  - 2014 Jan
TI  - Improvement in nerve regeneration through a decellularized nerve graft by 
      supplementation with bone marrow stromal cells in fibrin.
PG  - 97-110
LID - 10.3727/096368912X658845 [doi]
AB  - Acellular nerve grafting is often inferior as well as an inadequate alternative 
      to autografting for the repair of long gaps in peripheral nerves. Moreover, the 
      injection method is not perfect. During the injection of cells, the syringe can 
      destroy the acellular nerve structure and the limited accumulation of seed cells. 
      To resolve this problem, we constructed a nerve graft by acellular nerve 
      grafting. Bone marrow-mesenchymal stromal cells (BM-MSCs) were affixed with 
      fibrin glue and injected inside or around the graft, which was then used to 
      repair a 15-mm nerve defect in rats. The acellular nerve graft maintained its 
      structure and composition, and its tensile strength was decreased, as determined 
      by two-photon microscopy and a tensile testing device. In vitro, MSCs embedded in 
      fibrin glue survived and secreted growth factors such as nerve growth factor 
      (NGF) and brain-derived neurotrophic factor (BDNF). We repaired 15-mm 
      Sprague-Dawley rat sciatic nerve defects using this nerve graft construction, and 
      MSCs injected around the graft helped improve nerve regeneration and functional 
      recovery of peripheral nerve lesions as determined by functional analysis and 
      histology. Therefore, we conclude that supplying MSCs in fibrin glue around 
      acellular nerves is successful in maintaining the nerve structure and can support 
      nerve regeneration similar to the direct injection of MSCs into the acellular 
      nerve for long nerve defects but may avoid destroying the nerve graft. The 
      technique is simple and is another option for stem cell transplantation.
FAU - Zhao, Zhe
AU  - Zhao Z
FAU - Wang, Yu
AU  - Wang Y
FAU - Peng, Jiang
AU  - Peng J
FAU - Ren, Zhiwu
AU  - Ren Z
FAU - Zhang, Li
AU  - Zhang L
FAU - Guo, Quanyi
AU  - Guo Q
FAU - Xu, Wenjing
AU  - Xu W
FAU - Lu, Shibi
AU  - Lu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121101
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 9001-31-4 (Fibrin)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Fibrin
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Nerve Growth Factor/physiology
MH  - Nerve Regeneration/*physiology
MH  - Nerve Tissue/*transplantation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Transplantation, Autologous
EDAT- 2012/11/07 06:00
MHDA- 2014/10/31 06:00
CRDT- 2012/11/07 06:00
PHST- 2012/11/07 06:00 [entrez]
PHST- 2012/11/07 06:00 [pubmed]
PHST- 2014/10/31 06:00 [medline]
AID - ct0739zhao [pii]
AID - 10.3727/096368912X658845 [doi]
PST - ppublish
SO  - Cell Transplant. 2014 Jan;23(1):97-110. doi: 10.3727/096368912X658845. Epub 2012 
      Nov 1.