PMID- 2313097
OWN - NLM
STAT- MEDLINE
DCOM- 19900416
LR  - 20181130
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 144
IP  - 6
DP  - 1990 Mar 15
TI  - Secretion by human fibroblasts of monocyte chemoattractant protein-1, the product 
      of gene JE.
PG  - 2377-83
AB  - We recently purified human monocyte chemoattractant protein-1 (MCP-1) from 
      culture fluids of either human glioma cell lines or mitogen-stimulated human 
      peripheral blood mononuclear leukocytes. It has now been shown that MCP-1 is the 
      product of the gene JE, which was first recognized by its expression in 
      fibroblasts stimulated with platelet-derived growth factor (PDGF). We therefore 
      studied secretion of MCP-1 by three human fibroblast cell lines. Monocyte 
      chemotactic activity was found in culture fluids of all three lines after growth 
      to confluence in DMEM-10% FCS, and the amounts secreted per cell were comparable 
      for the three lines. The MRC-5 line was chosen for further study. Monocyte 
      chemotactic activity secretion by confluent MRC-5 cultures continued after a 
      switch to serum-free medium and was not inhibited by anti-PDGF antibody, 
      indicating that secretion may not have been caused by autocrine release of PDGF. 
      When concentrated serum-free MRC-5 culture fluid was injected into an HPLC gel 
      filtration column, only one chemotactic activity peak was observed, which was in 
      the same location as glioma-derived MCP-1. The activity was completely absorbed 
      out by an anti-MCP-1 affinity column, which indicates that all the chemotactic 
      activity in MRC-5 culture fluid was accounted for by MCP-1. PDGF caused a marked 
      increase in chemotactic activity over that found in serum-free culture fluid of 
      MRC-5 or 501T cells. Immunoprecipitation by anti-human MCP-1 showed two bands, 
      corresponding to the two forms of MCP-1 previously described (MCP-1 alpha and 
      beta); and the amounts increased in response to PDGF stimulation. Thus, the 
      reported increase in human fibroblast JE mRNA in response to PDGF-containing 
      serum stimulation is reflected in increased secretion of the MCP-1 gene product.
FAU - Yoshimura, T
AU  - Yoshimura T
AD  - Immunopathology Section, National Cancer Institute, Frederick, MD 21701.
FAU - Leonard, E J
AU  - Leonard EJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Glycoproteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 11089-65-9 (Tunicamycin)
SB  - IM
MH  - Biological Assay
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Fibroblasts/*metabolism
MH  - Glycoproteins/*metabolism
MH  - Glycosylation
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Weight
MH  - Monocytes/physiology
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Precipitin Tests
MH  - RNA, Messenger/genetics
MH  - Tunicamycin/pharmacology
EDAT- 1990/03/15 00:00
MHDA- 1990/03/15 00:01
CRDT- 1990/03/15 00:00
PHST- 1990/03/15 00:00 [pubmed]
PHST- 1990/03/15 00:01 [medline]
PHST- 1990/03/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1990 Mar 15;144(6):2377-83.