PMID- 23131070
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20211021
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 13
DP  - 2012 Nov 6
TI  - B chromosome in the beetle Coprophanaeus cyanescens (Scarabaeidae): emphasis in 
      the organization of repetitive DNA sequences.
PG  - 96
LID - 10.1186/1471-2156-13-96 [doi]
AB  - BACKGROUND: To contribute to the knowledge of coleopteran cytogenetics, 
      especially with respect to the genomic content of B chromosomes, we analyzed the 
      composition and organization of repetitive DNA sequences in the Coprophanaeus 
      cyanescens karyotype. We used conventional staining and the application of 
      fluorescence in situ hybridization (FISH) mapping using as probes C0t-1 DNA 
      fraction, the 18S and 5S rRNA genes, and the LOA-like non-LTR transposable 
      element (TE). RESULTS: The conventional analysis detected 3 individuals (among 50 
      analyzed) carrying one small metacentric and mitotically unstable B chromosome. 
      The FISH analysis revealed a pericentromeric block of C0t-1 DNA in the B 
      chromosome but no 18S or 5S rDNA clusters in this extra element. Using the 
      LOA-like TE probe, the FISH analysis revealed large pericentromeric blocks in 
      eight autosomal bivalents and in the B chromosome, and a pericentromeric block 
      extending to the short arm in one autosomal pair. No positive hybridization 
      signal was observed for the LOA-like element in the sex chromosomes. CONCLUSIONS: 
      The results indicate that the origin of the B chromosome is associated with the 
      autosomal elements, as demonstrated by the hybridization with C0t-1 DNA and the 
      LOA-like TE. The present study is the first report on the cytogenetic mapping of 
      a TE in coleopteran chromosomes. These TEs could have been involved in the origin 
      and evolution of the B chromosome in C. cyanescens.
FAU - Gomes de Oliveira, Sarah
AU  - Gomes de Oliveira S
AD  - Department of Morphology, Bioscience Institute, UNESP - Sao Paulo State 
      University, Botucatu, SP, 18618-970, Brazil.
FAU - Cassia de Moura, Rita
AU  - Cassia de Moura R
FAU - Martins, Cesar
AU  - Martins C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121106
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
RN  - 0 (DNA Transposable Elements)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes/*genetics
MH  - Coleoptera/*genetics
MH  - DNA Transposable Elements/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Metaphase
MH  - Multigene Family
MH  - RNA, Ribosomal, 18S/genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
PMC - PMC3506448
EDAT- 2012/11/08 06:00
MHDA- 2013/03/27 06:00
PMCR- 2012/11/06
CRDT- 2012/11/08 06:00
PHST- 2012/08/20 00:00 [received]
PHST- 2012/11/04 00:00 [accepted]
PHST- 2012/11/08 06:00 [entrez]
PHST- 2012/11/08 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
PHST- 2012/11/06 00:00 [pmc-release]
AID - 1471-2156-13-96 [pii]
AID - 10.1186/1471-2156-13-96 [doi]
PST - epublish
SO  - BMC Genet. 2012 Nov 6;13:96. doi: 10.1186/1471-2156-13-96.