PMID- 23132144
OWN - NLM
STAT- MEDLINE
DCOM- 20130912
LR  - 20131121
IS  - 1432-0703 (Electronic)
IS  - 0090-4341 (Linking)
VI  - 64
IP  - 2
DP  - 2013 Feb
TI  - A combination of micronucleus assay and fluorescence in situ hybridization 
      analysis to evaluate the genotoxicity of formaldehyde.
PG  - 337-44
LID - 10.1007/s00244-012-9828-6 [doi]
AB  - A genotoxic effect of formaldehyde (FA), particularly micronucleus (MN) 
      induction, has been shown in several previous studies. The aim of the present 
      study was to assess the frequency of micronuclei and to identify the type of 
      chromosomal damage in Tunisian staff members working in the Pathologic Anatomy 
      Laboratory of Farhat Hached hospital (Sousse, Tunisia) who were exposed to FA. 
      Assessment of chromosomal damage was performed in peripheral lymphocytes of 31 
      FA-exposed employees compared with 31 control employees working in the 
      administrative department of the same hospital. The clastogenic/aneugenic effect 
      of FA was evaluated using the standard MN assay in combination with fluorescence 
      in situ hybridization (FISH) using pan-centromeric probes. The mean level of 
      exposure to FA was 3.4 ppm. The results showed a significant increase of MN 
      frequency in lymphocytes of exposed workers compared with the control group 
      (25.35 +/- 6.28  per thousand vs. 7.08  +/- 4.62  per thousand, p < 0.05). As assessed by FISH, the frequency 
      of centromeric micronuclei (C+MN) was greater in exposed subjects than in 
      controls (18.38 +/- 5.94  per thousand vs. 5.03 +/- 3.64  per thousand). Among the C+MN, the frequency of MN 
      containing one centromere (C1+MN) was significantly greater in pathologists and 
      anatomists than in controls (15.35 +/- 6.0  per thousand vs. 3.33 +/- 2.74  per thousand, p < 0.05). The 
      results showed an effect of sex and time of FA exposure with significantly 
      increased frequencies of all end points measuring aneuploidy (C+MN, C1+MN, and 
      Cx+MN [more then one MN]). The increased frequency of C1+MN observed in the 
      exposed group may suggest a slight aneugenic effect of FA exposure.
FAU - Bouraoui, Sana
AU  - Bouraoui S
AD  - Department of Cytogenetic and Reproductive Biology, Farhat Hached University 
      Teaching Hospital, Ibn EL JAZZAR Street, 4000 Sousse, Tunisia. 
      sana_bouraoui@yahoo.fr
FAU - Mougou, Soumaya
AU  - Mougou S
FAU - Brahem, Aicha
AU  - Brahem A
FAU - Tabka, Faten
AU  - Tabka F
FAU - Ben Khelifa, Hela
AU  - Ben Khelifa H
FAU - Harrabi, Imed
AU  - Harrabi I
FAU - Mrizek, Najib
AU  - Mrizek N
FAU - Elghezal, Hatem
AU  - Elghezal H
FAU - Saad, Ali
AU  - Saad A
LA  - eng
PT  - Journal Article
DEP - 20121108
PL  - United States
TA  - Arch Environ Contam Toxicol
JT  - Archives of environmental contamination and toxicology
JID - 0357245
RN  - 0 (Air Pollutants, Occupational)
RN  - 0 (Mutagens)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Adult
MH  - Air Pollutants, Occupational/analysis/*toxicity
MH  - DNA Damage
MH  - Female
MH  - Formaldehyde/analysis/*toxicity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Micronucleus Tests
MH  - Middle Aged
MH  - Mutagens/analysis/*toxicity
MH  - Occupational Exposure/*adverse effects
MH  - Risk Assessment
EDAT- 2012/11/08 06:00
MHDA- 2013/09/13 06:00
CRDT- 2012/11/08 06:00
PHST- 2012/03/25 00:00 [received]
PHST- 2012/10/09 00:00 [accepted]
PHST- 2012/11/08 06:00 [entrez]
PHST- 2012/11/08 06:00 [pubmed]
PHST- 2013/09/13 06:00 [medline]
AID - 10.1007/s00244-012-9828-6 [doi]
PST - ppublish
SO  - Arch Environ Contam Toxicol. 2013 Feb;64(2):337-44. doi: 
      10.1007/s00244-012-9828-6. Epub 2012 Nov 8.