PMID- 23132797
OWN - NLM
STAT- MEDLINE
DCOM- 20130214
LR  - 20121204
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 53
IP  - 13
DP  - 2012 Dec 3
TI  - Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after 
      phacoemulsification.
PG  - 7951-60
LID - 10.1167/iovs.12-10231 [doi]
AB  - PURPOSE: To elucidate the impact of phacoemulsification on aqueous monocyte 
      chemoattractant protein-1 (MCP-1) levels, and identify its cell origin. METHODS: 
      For clinical study, aqueous humor samples were collected before and after surgery 
      (17.0 +/- 4.0 months postoperatively) from 21 cataract cases that underwent 
      phacoemulsification and intraocular lens (IOL) implantation. Aqueous MCP-1 levels 
      were determined using a multiplex immunoassay. For animal experiments, rabbits 
      underwent phacoemulsification (+/- IOL). Aqueous humor samples were collected from 
      nonoperated eyes and operated eyes, and immunoassays were performed. Eyes were 
      analyzed by reverse transcription-polymerase chain reaction and 
      immunohistochemical studies. RESULTS: In the clinical study, mean (+/- SD) aqueous 
      MCP-1 levels were higher postoperatively (1773.5 +/- 321.2 pg/mL) than 
      preoperatively (796.9 +/- 211.3 pg/mL; P < 0.0001). In animal experiments, mean 
      aqueous MCP-1 levels (pg/mL) were higher in postoperative eyes on day 30 (207.1 +/- 
      62.9) than in nonoperated eyes (31.2 +/- 12.5; P = 0.018). IOL implantation did not 
      affect the changes in MCP-1 levels. After phacoemulsification, MCP-1 mRNA 
      expression was increased in the cornea, iris, ciliary body, and capsular bag. 
      Expression of MCP-1 mRNA in the capsular bag, but not the other tissues, 
      increased from day 30 to 90. Immunohistochemical studies showed positive 
      immunoreactivity for MCP-1 in cells of the posterior capsule after 
      phacoemulsification. CONCLUSIONS: aqueous MCP-1 levels were elevated in both 
      human and animal eyes after phacoemulsification. Proliferated Lens epithelial 
      cells on the capsule might be the major cell origin for prolonged MCP-1 
      production after phacoemulsification. (http://www.umin.ac.jp/ number, 
      UMIN000005788.).
FAU - Kawai, Motofumi
AU  - Kawai M
AD  - Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, 
      Kumamoto, Japan.
FAU - Inoue, Toshihiro
AU  - Inoue T
FAU - Inatani, Masaru
AU  - Inatani M
FAU - Tsuboi, Naoko
AU  - Tsuboi N
FAU - Shobayashi, Kohei
AU  - Shobayashi K
FAU - Matsukawa, Akihiro
AU  - Matsukawa A
FAU - Yoshida, Akitoshi
AU  - Yoshida A
FAU - Tanihara, Hidenobu
AU  - Tanihara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121203
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (platelet-derived growth factor A)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Aqueous Humor/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Ciliary Body/metabolism
MH  - Cornea/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Iris/metabolism
MH  - Lens Capsule, Crystalline/metabolism
MH  - *Lens Implantation, Intraocular
MH  - Lens, Crystalline/cytology
MH  - Male
MH  - Middle Aged
MH  - *Phacoemulsification
MH  - Platelet-Derived Growth Factor/metabolism
MH  - Postoperative Period
MH  - RNA, Messenger/metabolism
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2012/11/08 06:00
MHDA- 2013/02/15 06:00
CRDT- 2012/11/08 06:00
PHST- 2012/11/08 06:00 [entrez]
PHST- 2012/11/08 06:00 [pubmed]
PHST- 2013/02/15 06:00 [medline]
AID - iovs.12-10231 [pii]
AID - 10.1167/iovs.12-10231 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2012 Dec 3;53(13):7951-60. doi: 10.1167/iovs.12-10231.