PMID- 23136039
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20160511
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 3
IP  - 5
DP  - 2013 May
TI  - Detecting local immunoglobulin E from mucosal brush biopsy of the inferior 
      turbinates using microarray analysis.
PG  - 399-403
LID - 10.1002/alr.21111 [doi]
AB  - BACKGROUND: It has been previously demonstrated that local, antigen-specific 
      immunoglobulin E (IgE) can be detected using a standard in vitro assay of lysed 
      epithelial cells in saline, harvested via nasal mucosal brush biopsy (MBB). 
      However, compared to surgical biopsy or serum, smaller amounts of IgE are 
      harvested using MBB, making detection much more difficult. Microarray analysis 
      (MA) requires less IgE for detection, making this an attractive option for MBB. 
      The goals of this study were to compare MA to a standard IgE assay for detecting 
      antigen-specific IgE from MBB and to test the association between the presence of 
      multiple positive components on MA with specific IgE on standard assay and 
      skin-prick testing (SPT) grade. METHODS: MBB samples from 18 allergic rhinitis 
      patients, which were previously tested for antigen-specific IgE to common 
      airborne allergens using a standard IgE assay, underwent MA for antigen-specific 
      IgE to multiple components of airborne and food allergens. Fisher's exact 
      probability testing was used to measure the strength of association between the 2 
      testing modalities for Timothy grass, ragweed, cat, Alternaria, and D. farinae. 
      RESULTS: MA correlated very highly with standard assay (p < 0.0001) and 50% of 
      positive antigens on MA detected multiple components to that antigen. The 
      presence of multiple components was not associated with specific IgE levels on 
      standard assay or SPT grade. CONCLUSION: This is the first demonstration that 
      antigen-specific IgE in saline samples can be measured using MA. The ability of 
      MA to measure smaller amounts of IgE, with similar accuracy, may give it a 
      potential advantage for MBB analysis in the future.
CI  - (c) 2013 ARS-AAOA, LLC.
FAU - Reisacher, William R
AU  - Reisacher WR
AD  - Department of Otolaryngology-Head and Neck Surgery, Weill Cornell Medical 
      College, NewYork-Presbyterian Hospital, 1305 York Avenue, New York, NY 10021, 
      USA. wir2011@med.cornell.edu
LA  - eng
PT  - Journal Article
DEP - 20121107
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/adverse effects/immunology
MH  - Biopsy
MH  - Enterocytes/*immunology/ultrastructure
MH  - Feasibility Studies
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Microarray Analysis/*methods
MH  - Microvilli/*metabolism/ultrastructure
MH  - Nasal Mucosa/*immunology/pathology
MH  - Pollen/adverse effects/immunology
MH  - Rhinitis, Allergic, Perennial/*diagnosis/immunology
MH  - Rhinitis, Allergic, Seasonal/*diagnosis/immunology
MH  - Turbinates/immunology/pathology
EDAT- 2012/11/09 06:00
MHDA- 2014/01/01 06:00
CRDT- 2012/11/09 06:00
PHST- 2012/07/27 00:00 [received]
PHST- 2012/09/18 00:00 [revised]
PHST- 2012/09/25 00:00 [accepted]
PHST- 2012/11/09 06:00 [entrez]
PHST- 2012/11/09 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - 10.1002/alr.21111 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2013 May;3(5):399-403. doi: 10.1002/alr.21111. Epub 
      2012 Nov 7.