PMID- 23136110
OWN - NLM
STAT- MEDLINE
DCOM- 20130805
LR  - 20220408
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 3
IP  - 2
DP  - 2013 Feb
TI  - Noninvasive Staphylococcus aureus biofilm determination in chronic rhinosinusitis 
      by detecting the exopolysaccharide matrix component poly-N-acetylglucosamine.
PG  - 83-8
LID - 10.1002/alr.21115 [doi]
AB  - BACKGROUND: The role that bacterial biofilms might play in recalcitrant forms of 
      chronic rhinosinusitis (CRS) is increasingly being recognized. However, the 
      detection of bacteria existing in this form, using standard culture, is limited 
      by their unique metabolically inactive properties. All current biofilm diagnostic 
      modalities require invasive mucosal biopsies, which limit their use to the 
      operating theatre. METHODS: Twenty CRS patients and 5 controls were enrolled in a 
      prospective study to assess the feasibility of noninvasively diagnosing S. aureus 
      biofilms by detecting the biofilm matrix polysaccharide poly-N-acetylglucosamine 
      (PNAG). An immunofluorescence protocol was developed for PNAG detection and 
      compared with both standard microbiological cultures and fluorescence in situ 
      hybridization (FISH). RESULTS: Thirteen of 20 CRS patients had evidence of S. 
      aureus biofilm formation using FISH. Of these, 12 had detectable PNAG. 
      Interestingly none of the S. aureus FISH-negative patients were PNAG-positive 
      despite the presence of coagulase-negative Staphylococci biofilms, some of which 
      may exhibit PNAG in their pathogenic forms. The development of a noninvasive S. 
      aureus biofilm diagnostic test provides a reliable means to identify a high-risk 
      group of CRS patients who harbor S. aureus biofilms. The ability to be used 
      outside of the perioperative period to assess surgical efficacy, guide 
      management, and evaluate new treatment modalities provides a significant advance 
      in this field of research and clinical practice. CONCLUSION: This study has 
      confirmed the feasibility of noninvasive detection of S. aureus biofilms with a 
      simple test that produces results comparable to the more invasive methods that 
      are currently relied upon.
CI  - (c) 2013 ARS-AAOA, LLC.
FAU - Foreman, Andrew
AU  - Foreman A
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Discipline of Surgery, 
      University of Adelaide and Flinders University, Adelaide, Australia.
FAU - Jervis-Bardy, Josh
AU  - Jervis-Bardy J
FAU - Boase, Samuel J
AU  - Boase SJ
FAU - Tan, Lorwai
AU  - Tan L
FAU - Wormald, Peter-John
AU  - Wormald PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121107
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.- (poly-N-acetylglucosamine deacetylase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amidohydrolases/*isolation & purification
MH  - Biofilms/*growth & development
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Rhinitis/*microbiology
MH  - Sinusitis/*microbiology
MH  - Staphylococcal Infections/*microbiology
MH  - Staphylococcus aureus/isolation & purification/*physiology
EDAT- 2012/11/09 06:00
MHDA- 2013/08/06 06:00
CRDT- 2012/11/09 06:00
PHST- 2012/05/20 00:00 [received]
PHST- 2012/08/29 00:00 [revised]
PHST- 2012/09/25 00:00 [accepted]
PHST- 2012/11/09 06:00 [entrez]
PHST- 2012/11/09 06:00 [pubmed]
PHST- 2013/08/06 06:00 [medline]
AID - 10.1002/alr.21115 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2013 Feb;3(2):83-8. doi: 10.1002/alr.21115. Epub 2012 
      Nov 7.