PMID- 23138187
OWN - NLM
STAT- MEDLINE
DCOM- 20130617
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 40
IP  - 2
DP  - 2013 Feb
TI  - Deletion of RBP-J in dendritic cells compromises TLR-mediated DC activation 
      accompanied by abnormal cytoskeleton reorganization.
PG  - 1531-9
LID - 10.1007/s11033-012-2198-3 [doi]
AB  - Dendritic cells (DCs) are professional antigen presenting cells that activate and 
      modulate immune responses, but the mechanisms underlying DC activation have not 
      been fully understood. In this study, we investigated the role of Notch signaling 
      in DC activation by using murine bone marrow-derived DCs. Triggering of Toll-like 
      receptors (TLRs) of DCs led to upregulated expression of Notch ligands. 
      Disruption of Notch signaling by the deletion of RBP-J, the critical 
      transcription factor mediating the canonical signaling from all Notch receptors, 
      resulted in a reduced capacity of DCs in activating T cells. Moreover, RBP-J 
      deficiency altered the polarization of T cell activation, as manifested by 
      downregulated interferon-gamma and upregulated interleukin-4 and -10 expressions 
      after LPS or Poly(I:C) stimulation. Furthermore, we found that RBP-J(-/-) DCs had 
      reduced intracellular calcium after TLR-triggering. Immunofluorescent staining 
      showed that RBP-J deficient DCs exhibited attenuated cytoskeleton reorganization 
      when contacting T cells. In summary, our results suggested that the canonical 
      Notch signaling promotes the cytoskeleton reorganization and the TLR-mediated DC 
      activation.
FAU - Chen, Yun-Ru
AU  - Chen YR
AD  - Department of Hematology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an, 710038, China.
FAU - Feng, Fan
AU  - Feng F
FAU - Wang, Li
AU  - Wang L
FAU - Qu, Shuo-Yao
AU  - Qu SY
FAU - Zhang, Zhen-Qiang
AU  - Zhang ZQ
FAU - Liu, Li
AU  - Liu L
FAU - Qin, Hong-Yan
AU  - Qin HY
FAU - Liang, Ying-Min
AU  - Liang YM
FAU - Han, Hua
AU  - Han H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121109
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DLL4 protein, mouse)
RN  - 0 (Dlk1 protein, mouse)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Immunoglobulin J Recombination Signal Sequence-Binding Protein)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Rbpj protein, mouse)
RN  - 0 (Receptors, Notch)
RN  - 0 (Serrate-Jagged Proteins)
RN  - 0 (Toll-Like Receptors)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium-Binding Proteins/genetics/metabolism
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Cytoskeletal Proteins/*metabolism
MH  - Dendritic Cells/immunology/*metabolism/ultrastructure
MH  - Gene Deletion
MH  - Immunoglobulin J Recombination Signal Sequence-Binding 
      Protein/deficiency/*genetics
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Intracellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Receptors, Notch/metabolism
MH  - Serrate-Jagged Proteins
MH  - Signal Transduction
MH  - T-Lymphocytes/physiology
MH  - Toll-Like Receptors/*metabolism
MH  - Up-Regulation
EDAT- 2012/11/10 06:00
MHDA- 2013/06/19 06:00
CRDT- 2012/11/10 06:00
PHST- 2012/05/07 00:00 [received]
PHST- 2012/10/09 00:00 [accepted]
PHST- 2012/11/10 06:00 [entrez]
PHST- 2012/11/10 06:00 [pubmed]
PHST- 2013/06/19 06:00 [medline]
AID - 10.1007/s11033-012-2198-3 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2013 Feb;40(2):1531-9. doi: 10.1007/s11033-012-2198-3. Epub 2012 
      Nov 9.