PMID- 23138943
OWN - NLM
STAT- MEDLINE
DCOM- 20130412
LR  - 20231213
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 957
DP  - 2013
TI  - Studying meiosis-specific cohesins in mouse embryonic oocytes.
PG  - 47-57
LID - 10.1007/978-1-62703-191-2_3 [doi]
AB  - Distinct meiotic cohesin complexes play fundamental roles in various 
      meiosis-specific chromosomal events in spatiotemporally different manners during 
      mammalian meiotic prophase. Immunostaining is one of the essential methods to 
      study meiotic cohesin dynamics. For the study of cohesins in the meiotic prophase 
      of oocytes, ovaries should be taken from the embryos during a very limited period 
      before birth. Here we focus on some technical tips concerning the preparation of 
      oocyte chromosome spreads for immunostaining. Further, we describe a method for 
      chromosome fluorescence in situ hybridization (FISH) against immunostained 
      oocytes.
FAU - Kim, Ji-hye
AU  - Kim JH
AD  - Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular 
      Biosciences, University of Tokyo, Tokyo, Japan.
FAU - Ishiguro, Kei-ichiro
AU  - Ishiguro K
FAU - Kudo, Nobuaki
AU  - Kudo N
FAU - Watanabe, Yoshinori
AU  - Watanabe Y
LA  - eng
GR  - G0901046/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*metabolism
MH  - Centrifugation
MH  - Chromosomal Proteins, Non-Histone/*metabolism
MH  - Chromosomes, Mammalian/genetics
MH  - Cytogenetic Analysis/*methods
MH  - Embryo, Mammalian/*cytology
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis/*genetics
MH  - Mice
MH  - Oocytes/*cytology/*metabolism
MH  - Ovary/cytology/embryology
MH  - Prophase/genetics
MH  - Cohesins
EDAT- 2012/11/10 06:00
MHDA- 2013/04/13 06:00
CRDT- 2012/11/10 06:00
PHST- 2012/11/10 06:00 [entrez]
PHST- 2012/11/10 06:00 [pubmed]
PHST- 2013/04/13 06:00 [medline]
AID - 10.1007/978-1-62703-191-2_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;957:47-57. doi: 10.1007/978-1-62703-191-2_3.