PMID- 23139176
OWN - NLM
STAT- MEDLINE
DCOM- 20131205
LR  - 20231213
IS  - 1349-9157 (Electronic)
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 54
IP  - 2
DP  - 2013 Mar 1
TI  - Human cell responses to ionizing radiation are differentially affected by the 
      expressed connexins.
PG  - 251-9
LID - 10.1093/jrr/rrs099 [doi]
AB  - In multicellular organisms, intercellular communication is essential for 
      homeostatic functions and has a major role in tissue responses to stress. Here, 
      we describe the effects of expression of different connexins, which form gap 
      junction channels with different permeabilities, on the responses of human cells 
      to ionizing radiation. Exposure of confluent HeLa cell cultures to (137)Cs gamma 
      rays, 3.7 MeV alpha particles, 1000 MeV protons or 1000 MeV/u iron ions resulted in 
      distinct effects when the cells expressed gap junction channels composed of 
      either connexin26 (Cx26) or connexin32 (Cx32). Irradiated HeLa cells expressing 
      Cx26 generally showed decreased clonogenic survival and reduced metabolic 
      activity relative to parental cells lacking gap junction communication. In 
      contrast, irradiated HeLa cells expressing Cx32 generally showed enhanced 
      survival and greater metabolic activity relative to the control cells. The 
      effects on clonogenic survival correlated more strongly with effects on metabolic 
      activity than with DNA damage as assessed by micronucleus formation. The data 
      also showed that the ability of a connexin to affect clonogenic survival 
      following ionizing radiation can depend on the specific type of radiation. 
      Together, these findings show that specific types of connexin channels are 
      targets that may be exploited to enhance radiotherapeutic efficacy and to 
      formulate countermeasures to the harmful effects of specific types of ionizing 
      radiation.
FAU - Autsavapromporn, Narongchai
AU  - Autsavapromporn N
AD  - Department of Radiology, New Jersey Medical School Cancer Center, Newark NJ 07103 
      USA.
FAU - De Toledo, Sonia M
AU  - De Toledo SM
FAU - Jay-Gerin, Jean-Paul
AU  - Jay-Gerin JP
FAU - Harris, Andrew L
AU  - Harris AL
FAU - Azzam, Edouard I
AU  - Azzam EI
LA  - eng
GR  - CA049062/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20121108
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
RN  - 0 (Connexins)
RN  - 0 (GJB2 protein, human)
RN  - 127120-53-0 (Connexin 26)
SB  - IM
MH  - Bystander Effect/*physiology/*radiation effects
MH  - Cell Survival/*physiology/radiation effects
MH  - Connexin 26
MH  - Connexins/*metabolism
MH  - DNA Damage/*physiology
MH  - Dose-Response Relationship, Radiation
MH  - Gene Expression Regulation/physiology/radiation effects
MH  - HeLa Cells
MH  - Humans
MH  - Radiation Dosage
MH  - Signal Transduction/physiology/radiation effects
MH  - Gap Junction beta-1 Protein
PMC - PMC3589937
EDAT- 2012/11/10 06:00
MHDA- 2013/12/16 06:00
PMCR- 2012/11/08
CRDT- 2012/11/10 06:00
PHST- 2012/11/10 06:00 [entrez]
PHST- 2012/11/10 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2012/11/08 00:00 [pmc-release]
AID - rrs099 [pii]
AID - 10.1093/jrr/rrs099 [doi]
PST - ppublish
SO  - J Radiat Res. 2013 Mar 1;54(2):251-9. doi: 10.1093/jrr/rrs099. Epub 2012 Nov 8.