PMID- 23140616
OWN - NLM
STAT- MEDLINE
DCOM- 20140204
LR  - 20190911
IS  - 1875-5305 (Electronic)
IS  - 0929-8665 (Linking)
VI  - 20
IP  - 7
DP  - 2013 Jul 1
TI  - Expression of short peptide by an improved isocaudamer tandem repeat strategy.
PG  - 808-12
AB  - An improved isocaudamer tandem repeat strategy for the production of short 
      functional peptide was demonstrated in the study. The coding sequence of short 
      peptide was codon optimized, and two isocaudamers were induced into the end of 
      coding sequence. By re-cutting with isocaudamers and re-ligating, the coding 
      sequence of short peptide in the expression vector was increased in a multiple 
      manner (21, 22, 23, 24, 25 .......). In the present study, an 8 amino-acidresidue 
      peptide of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      effectively expressed in 8 copies and 16 copies by this approach, then the 
      proteins in 8 copies and 16 copies were used to generate antibody against this 
      epitope in rabbits. The results showed that PRRSVs were well recognized by the 
      antibody in indirect immunofluorescence assay. The technology using isocaudamer 
      to insert multiple tandem repeats in the vector provides an important approach 
      for the studies of small molecule peptides.
FAU - An, Tong-Qing
AU  - An TQ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
FAU - Leng, Chao-Liang
AU  - Leng CL
FAU - Yang, Yong-Qian
AU  - Yang YQ
FAU - Peng, Jin-Mei
AU  - Peng JM
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Protein Pept Lett
JT  - Protein and peptide letters
JID - 9441434
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Oligopeptides)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology/metabolism
MH  - Cell Line
MH  - Cloning, Molecular/*methods
MH  - Epitopes/immunology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Oligopeptides/*biosynthesis/genetics/immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/metabolism
MH  - Rabbits
MH  - Recombinant Proteins/biosynthesis/genetics/immunology
MH  - *Tandem Repeat Sequences
EDAT- 2012/11/13 06:00
MHDA- 2014/02/05 06:00
CRDT- 2012/11/13 06:00
PHST- 2012/07/21 00:00 [received]
PHST- 2012/10/31 00:00 [revised]
PHST- 2012/10/31 00:00 [accepted]
PHST- 2012/11/13 06:00 [entrez]
PHST- 2012/11/13 06:00 [pubmed]
PHST- 2014/02/05 06:00 [medline]
AID - PPL-EPUB-20121105-1 [pii]
AID - 10.2174/0929866511320070011 [doi]
PST - ppublish
SO  - Protein Pept Lett. 2013 Jul 1;20(7):808-12. doi: 10.2174/0929866511320070011.