PMID- 23140662 OWN - NLM STAT- MEDLINE DCOM- 20130503 LR - 20121203 IS - 1618-0984 (Electronic) IS - 0723-2020 (Linking) VI - 35 IP - 8 DP - 2012 Dec TI - Crystal ball: fluorescence in situ hybridization in the age of super-resolution microscopy. PG - 549-52 LID - S0723-2020(12)00125-7 [pii] LID - 10.1016/j.syapm.2012.10.001 [doi] AB - Super-resolution microscopy encompasses a suite of cutting edge microscopy methods able to surpass the resolution limits of light microscopy. The recent commercial availability of super-resolution microscopy is advancing many fields of biology. In this crystal ball forward look, we briefly examine the perspectives of combining super-resolution microscopy and fluorescence in situ hybridization (FISH). We strongly believe, based on first evidence presented here, that using super-resolution microscopy in environmental microbiology has the potential to reshape the way we analyze the results obtained with FISH, by improving both the localization and quantification of target molecules. CI - Copyright (c) 2012 Elsevier GmbH. All rights reserved. FAU - Moraru, Cristina AU - Moraru C AD - Max Planck Institute for Marine Microbiology, Celssiusstrasse 1, 28359 Bremen, Germany. CristinaMoraru@TheScientist.info FAU - Amann, Rudolf AU - Amann R LA - eng PT - Journal Article DEP - 20121107 PL - Germany TA - Syst Appl Microbiol JT - Systematic and applied microbiology JID - 8306133 SB - IM MH - *Environmental Microbiology MH - In Situ Hybridization, Fluorescence/*methods MH - Microbiology/trends MH - Microscopy/*methods EDAT- 2012/11/13 06:00 MHDA- 2013/05/04 06:00 CRDT- 2012/11/13 06:00 PHST- 2012/09/06 00:00 [received] PHST- 2012/10/03 00:00 [revised] PHST- 2012/10/05 00:00 [accepted] PHST- 2012/11/13 06:00 [entrez] PHST- 2012/11/13 06:00 [pubmed] PHST- 2013/05/04 06:00 [medline] AID - S0723-2020(12)00125-7 [pii] AID - 10.1016/j.syapm.2012.10.001 [doi] PST - ppublish SO - Syst Appl Microbiol. 2012 Dec;35(8):549-52. doi: 10.1016/j.syapm.2012.10.001. Epub 2012 Nov 7.