PMID- 23141928
OWN - NLM
STAT- MEDLINE
DCOM- 20130606
LR  - 20220408
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 182
IP  - 1
DP  - 2013 Jan
TI  - Long intergenic noncoding RNA HOTAIR is overexpressed and regulates PTEN 
      methylation in laryngeal squamous cell carcinoma.
PG  - 64-70
LID - S0002-9440(12)00716-X [pii]
LID - 10.1016/j.ajpath.2012.08.042 [doi]
AB  - Homeobox (HOX) transcript antisense RNA (HOTAIR) is a long intergenic noncoding 
      RNA (lincRNA) that is significantly overexpressed in breast and hepatocellular 
      cancers. It remains unclear, however, whether HOTAIR plays an oncogenic role in 
      human laryngeal squamous cell cancer (LSCC). We therefore investigated the 
      expression and functional role of HOTAIR in LSCC. HOTAIR levels were 
      significantly higher in LSCC than in corresponding adjacent non-neoplastic 
      tissues, and patients with poor histological grade or advanced clinical stage had 
      higher HOTAIR expression. Log-rank test showed a significant association between 
      high levels of HOTAIR and poor prognosis in LSCC patients. Multivariate Cox 
      analysis suggested that HOTAIR is an independent prognostic factor of LSCC. 
      siRNA-mediated knockdown of HOTAIR led to reduced invasion and increased 
      apoptosis of Hep-2 cells in vitro and significantly reduced growth of LSCC 
      xenograft tumors in mice. Moreover, PTEN methylation was significantly reduced in 
      Hep-2 cells depleted of HOTAIR. Taken together, these results suggest that the 
      oncogenic role of HOTAIR in LSCC is related to promotion of PTEN methylation. 
      HOTAIR could serve as a marker for LSCC prognosis and a potential target for 
      therapeutic intervention.
CI  - Copyright (c) 2013 American Society for Investigative Pathology. Published by 
      Elsevier Inc. All rights reserved.
FAU - Li, Dandan
AU  - Li D
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, China.
FAU - Feng, Jiapeng
AU  - Feng J
FAU - Wu, Tianyi
AU  - Wu T
FAU - Wang, Yandong
AU  - Wang Y
FAU - Sun, Yanan
AU  - Sun Y
FAU - Ren, Jingyuan
AU  - Ren J
FAU - Liu, Ming
AU  - Liu M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121107
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Neoplasm)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Biomarkers, Tumor/biosynthesis/genetics
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - DNA Methylation/genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Knockdown Techniques
MH  - Genes, Neoplasm
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Transplantation
MH  - PTEN Phosphohydrolase/*genetics/metabolism
MH  - Prognosis
MH  - RNA, Long Noncoding/*biosynthesis/genetics
MH  - RNA, Neoplasm/genetics
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 2012/11/13 06:00
MHDA- 2013/06/07 06:00
CRDT- 2012/11/13 06:00
PHST- 2012/01/14 00:00 [received]
PHST- 2012/07/03 00:00 [revised]
PHST- 2012/08/23 00:00 [accepted]
PHST- 2012/11/13 06:00 [entrez]
PHST- 2012/11/13 06:00 [pubmed]
PHST- 2013/06/07 06:00 [medline]
AID - S0002-9440(12)00716-X [pii]
AID - 10.1016/j.ajpath.2012.08.042 [doi]
PST - ppublish
SO  - Am J Pathol. 2013 Jan;182(1):64-70. doi: 10.1016/j.ajpath.2012.08.042. Epub 2012 
      Nov 7.