PMID- 23142721
OWN - NLM
STAT- MEDLINE
DCOM- 20130523
LR  - 20151119
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 533
DP  - 2013 Jan 15
TI  - Electrical stimulation enhances neurogenin2 expression through beta-catenin 
      signaling pathway of mouse bone marrow stromal cells and intensifies the effect 
      of cell transplantation on brain injury.
PG  - 71-6
LID - S0304-3940(12)01391-2 [pii]
LID - 10.1016/j.neulet.2012.10.023 [doi]
AB  - Bone marrow stromal cells (BMSCs) have received significant attention for its use 
      in neural regeneration. However, neural replacement by transplanted BMSCs was not 
      very effective. Recently, the gene transfection method has improved the 
      capability of cell transplantation; however, this method results in canceration 
      and immune rejection. We induced the differentiation of mouse BMSCs into neural 
      cells using electrical stimulation and transplanted the cells into traumatic 
      brain injury (TBI) model mice. We found that the electrically stimulated cells 
      have good potential to differentiate into neural cells and contribute to recovery 
      from TBI without differentiating into astrocytes. In addition, we found that 
      electrical stimulation enhanced neurogenin2 (Ngn2) expression. Ngn2 is involved 
      in neural differentiation and inhibits astrocytic differentiation during cell 
      growth. Furthermore, we found that this enhancement of Ngn2 expression occurred 
      through beta-catenin signaling pathway. This study may contribute to the use of 
      BMSCs for neural replacement in central nervous system diseases.
CI  - Copyright (c) 2012 Elsevier Ireland Ltd. All rights reserved.
FAU - Matsumoto, Masaya
AU  - Matsumoto M
AD  - Division of Bio-Environmental Adaptation Sciences, Graduate School of Biomedical 
      and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Imura, Takeshi
AU  - Imura T
FAU - Fukazawa, Takahiro
AU  - Fukazawa T
FAU - Sun, Yanan
AU  - Sun Y
FAU - Takeda, Masaaki
AU  - Takeda M
FAU - Kajiume, Teruyuki
AU  - Kajiume T
FAU - Kawahara, Yumi
AU  - Kawahara Y
FAU - Yuge, Louis
AU  - Yuge L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121106
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Biomarkers)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurog2 protein, mouse)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - *Bone Marrow Transplantation
MH  - Brain Injuries/pathology/physiopathology/*therapy
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Electric Stimulation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Movement
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/cytology/metabolism
MH  - Signal Transduction
MH  - Stromal Cells/cytology/metabolism/transplantation
MH  - beta Catenin/*physiology
EDAT- 2012/11/13 06:00
MHDA- 2013/05/25 06:00
CRDT- 2012/11/13 06:00
PHST- 2012/09/02 00:00 [received]
PHST- 2012/10/07 00:00 [revised]
PHST- 2012/10/19 00:00 [accepted]
PHST- 2012/11/13 06:00 [entrez]
PHST- 2012/11/13 06:00 [pubmed]
PHST- 2013/05/25 06:00 [medline]
AID - S0304-3940(12)01391-2 [pii]
AID - 10.1016/j.neulet.2012.10.023 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 Jan 15;533:71-6. doi: 10.1016/j.neulet.2012.10.023. Epub 2012 
      Nov 6.