PMID- 23144129
OWN - NLM
STAT- MEDLINE
DCOM- 20130529
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 79
IP  - 2
DP  - 2013 Jan
TI  - Characterization of a newly discovered symbiont of the whitefly Bemisia tabaci 
      (Hemiptera: Aleyrodidae).
PG  - 569-75
LID - 10.1128/AEM.03030-12 [doi]
AB  - Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 
      cryptic species, some of which are important crop pests worldwide. Like many 
      other sap-sucking insects, whiteflies harbor an obligatory symbiont, "Candidatus 
      Portiera aleyrodidarum," and a number of secondary symbionts. So far, six genera 
      of secondary symbionts have been identified in B. tabaci. In this study, we 
      report and describe the finding of an additional bacterium in the indigenous B. 
      tabaci cryptic species China 1 (formerly known as B. tabaci biotype ZHJ3). 
      Phylogenetic analysis based on the 16S rRNA and gltA genes showed that the 
      bacterium belongs to the Alphaproteobacteria subdivision of the Proteobacteria 
      and has a close relationship with human pathogens of the genus Orientia. 
      Consequently, we temporarily named it Orientia-like organism (OLO). OLO was found 
      in six of eight wild populations of B. tabaci China 1, with the infection rate 
      ranging from 46.2% to 76.8%. Fluorescence in situ hybridization (FISH) of B. 
      tabaci China 1 in nymphs and adults revealed that OLOs are confined to the 
      bacteriome and co-occur with "Ca. Portiera aleyrodidarum." The vertical 
      transmission of OLO was demonstrated by detection of OLO at the anterior pole end 
      of the oocytes through FISH. Quantitative PCR analysis of population dynamics 
      suggested a complex interaction between "Ca. Portiera aleyrodidarum" and OLO. 
      Based on these results, we propose "Candidatus Hemipteriphilus asiaticus" for the 
      classification of this symbiont from B. tabaci.
FAU - Bing, Xiao-Li
AU  - Bing XL
AD  - Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of 
      Insect Sciences, Zhejiang University, Hangzhou, China.
FAU - Yang, Jiao
AU  - Yang J
FAU - Zchori-Fein, Einat
AU  - Zchori-Fein E
FAU - Wang, Xiao-Wei
AU  - Wang XW
FAU - Liu, Shu-Sheng
AU  - Liu SS
LA  - eng
SI  - GENBANK/GQ303180
SI  - GENBANK/JX042434
SI  - GENBANK/JX042435
SI  - GENBANK/JX042436
SI  - GENBANK/JX042437
SI  - GENBANK/JX042438
SI  - GENBANK/JX042439
SI  - GENBANK/JX042440
SI  - GENBANK/JX042441
SI  - GENBANK/JX042442
SI  - GENBANK/JX042443
SI  - GENBANK/JX042444
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121109
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.4.1.13 (Glutamate Synthase)
SB  - IM
MH  - Alphaproteobacteria/genetics/*isolation & purification/*physiology
MH  - Animals
MH  - China
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Glutamate Synthase/genetics
MH  - Hemiptera/*microbiology/*physiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - *Symbiosis
PMC - PMC3553764
EDAT- 2012/11/13 06:00
MHDA- 2013/05/31 06:00
PMCR- 2013/07/01
CRDT- 2012/11/13 06:00
PHST- 2012/11/13 06:00 [entrez]
PHST- 2012/11/13 06:00 [pubmed]
PHST- 2013/05/31 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - AEM.03030-12 [pii]
AID - 03030-12 [pii]
AID - 10.1128/AEM.03030-12 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2013 Jan;79(2):569-75. doi: 10.1128/AEM.03030-12. Epub 
      2012 Nov 9.