PMID- 23146336
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20181202
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 22
IP  - 12
DP  - 2013
TI  - Angiogenesis following cell injection is induced by an excess inflammatory 
      response coordinated by bone marrow cells.
PG  - 2381-92
LID - 10.3727/096368912X658863 [doi]
AB  - The aim of this study was to identify novel angiogenic mechanisms underlying the 
      regenerative process. To that end, interactions between adipose tissue-derived 
      stromal cells (ASCs) and bone marrow cells (BMCs) were initially investigated 
      using real-time fluorescence optical imaging. To monitor cell behavior in mice, 
      we injected green fluorescent protein-positive (GFP(+)) BMCs into the tail vein 
      and injected PKH26-labeled ASCs behind the ears. Angiogenesis and inflammation 
      were observed at these sites via an optical imaging probe. Injected GFP(+) BMCs 
      migrated from the blood vessels into the tissues surrounding the ASC injection 
      sites. Many of the migrating GFP(+) BMCs discovered at the ASC injection sites 
      were inflammatory cells, including Gr-1(+), CD11b(+), and F4/80(+) cells. ASCs 
      cocultured with inflammatory cells secreted increased levels of chemokines such 
      as macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, keratinocyte-derived 
      chemokines, and monocyte chemotactic protein 1. Similarly, these ASCs secreted 
      increased levels of angiogenic growth factors such as hepatocyte growth factor 
      and vascular endothelial growth factor. However, when anti-CXC chemokine receptor 
      type 4 antibody was injected at regular intervals, the migration of GFP(+) BMCs 
      (especially Gr-1(+) and CD11b(+) cells) to ASC injection sites was inhibited, as 
      was angiogenesis. The collective influence of the injected ASCs and BMC-derived 
      inflammatory cells promoted acute inflammation and angiogenesis. Together, the 
      results suggest that the outcome of cell-based angiogenic therapy is influenced 
      not only by the injected cells but also by the effect of intrinsic inflammatory 
      cells.
FAU - Hattori, Hidemi
AU  - Hattori H
AD  - Division of Biomedical Engineering, Research Institute, National Defense Medical 
      College, Saitama, Japan.
FAU - Amano, Yoshiko
AU  - Amano Y
FAU - Habu-Ogawa, Yoshiko
AU  - Habu-Ogawa Y
FAU - Ando, Takahiro
AU  - Ando T
FAU - Takase, Bonpei
AU  - Takase B
FAU - Ishihara, Masayuki
AU  - Ishihara M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121108
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (CXCR4 protein, mouse)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*immunology/metabolism
MH  - *Bone Marrow Transplantation
MH  - Cell Movement/drug effects
MH  - Ear/blood supply
MH  - Hepatocyte Growth Factor/metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Neovascularization, Physiologic/drug effects
MH  - Receptors, CXCR4/immunology/metabolism
MH  - Tail/blood supply
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2012/11/14 06:00
MHDA- 2014/08/30 06:00
CRDT- 2012/11/14 06:00
PHST- 2012/11/14 06:00 [entrez]
PHST- 2012/11/14 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - ct0768hattori [pii]
AID - 10.3727/096368912X658863 [doi]
PST - ppublish
SO  - Cell Transplant. 2013;22(12):2381-92. doi: 10.3727/096368912X658863. Epub 2012 
      Nov 8.