PMID- 23149719
OWN - NLM
STAT- MEDLINE
DCOM- 20130605
LR  - 20211021
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 351
IP  - 1
DP  - 2013 Jan
TI  - Developmental changes in the responsiveness of rat spiral ganglion neurons to 
      neurotrophic factors in dissociated culture: differential responses for survival, 
      neuritogenesis and neuronal morphology.
PG  - 15-27
LID - 10.1007/s00441-012-1526-1 [doi]
AB  - The way that the development of the inner ear innervation is regulated by various 
      neurotrophic factors and/or their combinations at different postnatal 
      developmental stages remains largely unclear. Moreover, survival and 
      neuritogenesis in deafferented adult neurons is important for cochlear implant 
      function. To address these issues, developmental changes in the responsiveness of 
      postnatal rat spiral ganglion neurons (SGNs) to neurotrophin-3 (NT-3), 
      brain-derived neurotrophic factor (BDNF) and leukemia inhibitory factor (LIF) 
      were examined by using a dissociated cell culture system. SGNs at postnatal day 
      (P) 0, P5 and P20 (young adult) were cultured with the addition of NT-3, BDNF, or 
      LIF or of a combination of NT-3 and BDNF (N + B) or of NT-3, BDNF and LIF (ALL 
      factors). SGNs were analyzed for three parameters: survival, longest neurite 
      length (LNL) and neuronal morphology. At P0, SGNs required exposure to N + B or 
      ALL factors for enhanced survival and the ALL factors combination showed a 
      synergistic effect much greater than the sum of the individual factors. At P5, 
      SGNs responded to a wider range of treatment conditions for enhanced survival and 
      combinations showed only an additive improvement over individual factors. The 
      survival percentage of untreated SGNs was highest at P20 but combinations of 
      neurotrophic factors were no more effective than individual factors. LNL of each 
      SGN was enhanced by LIF alone or ALL factors at P0 and P5 but was suppressed by 
      NT-3, BDNF and N + B at P5 in a dose-dependent manner. The LNL at P20 was 
      enhanced by ALL factors and suppressed by N + B. Treatment with ALL factors 
      increased the proportion of SGNs that had two or more primary neurites in all age 
      groups. These findings suggest that NT-3, BDNF, LIF and their combinations 
      predominantly support different ontogenetic events at different developmental 
      stages in the innervation of the inner ear.
FAU - Jin, Yulian
AU  - Jin Y
AD  - Department of Otolaryngology and Head and Neck Surgery, Graduate School of 
      Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
FAU - Kondo, Kenji
AU  - Kondo K
FAU - Ushio, Munetaka
AU  - Ushio M
FAU - Kaga, Kimitaka
AU  - Kaga K
FAU - Ryan, Allen F
AU  - Ryan AF
FAU - Yamasoba, Tatsuya
AU  - Yamasoba T
LA  - eng
GR  - I01 BX001205/BX/BLRD VA/United States
GR  - R01 DC000129/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20121113
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Culture Techniques
MH  - Cell Shape/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Leukemia Inhibitory Factor/pharmacology
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/drug effects/*metabolism
MH  - Neurogenesis/*drug effects
MH  - Neurotrophin 3/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spiral Ganglion/*growth & development
PMC - PMC3577061
MID - NIHMS438940
EDAT- 2012/11/15 06:00
MHDA- 2013/06/06 06:00
PMCR- 2013/08/01
CRDT- 2012/11/15 06:00
PHST- 2012/08/13 00:00 [received]
PHST- 2012/10/24 00:00 [accepted]
PHST- 2012/11/15 06:00 [entrez]
PHST- 2012/11/15 06:00 [pubmed]
PHST- 2013/06/06 06:00 [medline]
PHST- 2013/08/01 00:00 [pmc-release]
AID - 10.1007/s00441-012-1526-1 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2013 Jan;351(1):15-27. doi: 10.1007/s00441-012-1526-1. Epub 2012 
      Nov 13.