PMID- 23154768
OWN - NLM
STAT- MEDLINE
DCOM- 20130121
LR  - 20191210
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 36
IP  - 12
DP  - 2012 Dec
TI  - Silver-enhanced in situ hybridization for determination of EGFR copy number 
      alterations in non-small cell lung cancer.
PG  - 1801-8
LID - 10.1097/PAS.0b013e31826ff153 [doi]
AB  - Epidermal growth factor receptor (EGFR) gene mutation and high gene copy number 
      (CN) predict response to EGFR tyrosine kinase inhibitor therapy in the 
      adenocarcinoma subtype of non-small cell lung cancer (NSCLC). The aims of this 
      study were first to compare automated enzyme metallographic silver-enhanced in 
      situ hybridization (SISH) with conventionally used fluorescence in situ 
      hybridization (FISH) in the determination of EGFR CN in NSCLC tissue sections, 
      and second to assess the association of EGFR CN with EGFR mutations and 
      clinicopathological parameters. FISH and SISH were performed on tissue 
      microarrays and large sections. Samples from 56 consecutively surgically resected 
      NSCLC patients (cohort 1) and from 60 selected lung adenocarcinoma patients 
      (cohort 2) were analyzed. EGFR CN was classified applying the Colorado criteria, 
      and agreement between both methods was evaluated using kappa values. EGFR CN was 
      compared with EGFR protein expression and EGFR gene mutations. The results of 
      SISH and FISH were identical in 114 of the 116 cases examined using the 2 
      techniques. One case was FISH+, SISH-, and 1 case was FISH- and SISH+. The 
      agreement between the 2 methods was good in cohort 1 (kappa=0.642 [0.428, 0.823]) and 
      excellent in cohort 2 (kappa=0.963 [0.870, 1.000]). EGFR positivity by FISH and SISH 
      was associated with high EGFR protein expression (P<0.001) and EGFR mutation 
      (P<0.001). These results validate the use of SISH for assessing EGFR CN 
      alterations in NSCLC. The advantage of a permanent result and the possibility of 
      bright-field microscopy make SISH an attractive alternative to FISH.
FAU - Wulf, Marie-Angela
AU  - Wulf MA
AD  - Institute of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland.
FAU - Bode, Beata
AU  - Bode B
FAU - Zimmermann, Dieter
AU  - Zimmermann D
FAU - Rufibach, Kaspar
AU  - Rufibach K
FAU - Weder, Walter
AU  - Weder W
FAU - Moch, Holger
AU  - Moch H
FAU - Soltermann, Alex
AU  - Soltermann A
FAU - Tischler, Verena
AU  - Tischler V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Acetates)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Silver Compounds)
RN  - 19PPS85F9H (silver acetate)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - *Acetates
MH  - Aged
MH  - Automation, Laboratory
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Carcinoma, Non-Small-Cell Lung/enzymology/*genetics/pathology
MH  - *DNA Copy Number Variations
MH  - DNA Mutational Analysis
MH  - ErbB Receptors/analysis/*genetics
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/enzymology/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - *Silver Compounds
MH  - Tissue Array Analysis
EDAT- 2012/11/17 06:00
MHDA- 2013/01/23 06:00
CRDT- 2012/11/17 06:00
PHST- 2012/11/17 06:00 [entrez]
PHST- 2012/11/17 06:00 [pubmed]
PHST- 2013/01/23 06:00 [medline]
AID - 00000478-201212000-00007 [pii]
AID - 10.1097/PAS.0b013e31826ff153 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2012 Dec;36(12):1801-8. doi: 10.1097/PAS.0b013e31826ff153.