PMID- 23155275
OWN - NLM
STAT- MEDLINE
DCOM- 20130304
LR  - 20151119
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 32
IP  - 11
DP  - 2012 Nov
TI  - c-MYC amplification in mucinous gastric carcinoma: a possible genetic alteration 
      leading to deeply invasive tumors.
PG  - 5031-7
AB  - BACKGROUND: Patients with mucinous gastric carcinoma (MGC) usually have a poor 
      prognosis, largely due to the advanced stage of disease. In this study, we 
      evaluated the effects of c-MYC amplification on tumor stage and disease-specific 
      survival of 128 patients with MGC and compared the results with those of 302 
      patients with non-mucinous gastric carcinoma (non-MGC). PATIENTS AND METHODS: 
      Two-color fluorescence in situ hybridization (FISH) for c-MYC was performed on 
      430 GC samples. Real-time quantitative polymerase chain reaction (q-PCR) analysis 
      for c-MYC was also performed after tumor microdissection. RESULTS: c-MYC 
      amplification was found in 10.2% of MGCs and 6.0% of non-MGCs. c-MYC 
      amplification was more frequently found in MGCs of higher tumor stage than in 
      MGCs of lower stage (p=0.038). c-MYC amplification in MGC was correlated with 
      greater invasion depth (p=0.007). The mean survival time of patients with c-MYC 
      amplification was shorter than that of patients without c-MYC amplification in 
      MGC. Real-time q-PCR results showed that the calculated c-MYC/GAPDH ratios were 
      higher in c-MYC-amplified MGC than in c-MYC-non-amplified MGC. CONCLUSION: This 
      study showed that c-MYC amplification in MGC is highly correlated with advanced 
      stage and deeply invasive MGC. This suggests that c-MYC amplification in MGC 
      could be a possible genetic alteration contributing to the frequent presentation 
      of advanced-stage MGC.
FAU - Choi, Jong-Sun
AU  - Choi JS
AD  - Department of Pathology, Dongguk University, Ilsan Hospital, Goyang, South Korea.
FAU - Seo, Jinwon
AU  - Seo J
FAU - Jung, Eun Ji
AU  - Jung EJ
FAU - Kim, Eo Jin
AU  - Kim EJ
FAU - Lee, Geon Kook
AU  - Lee GK
FAU - Kim, Woo Ho
AU  - Kim WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adenocarcinoma, Mucinous/*genetics/mortality/pathology
MH  - Biomarkers, Tumor/analysis/genetics
MH  - *Gene Amplification
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kaplan-Meier Estimate
MH  - Microdissection
MH  - Neoplasm Staging
MH  - Proportional Hazards Models
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stomach Neoplasms/*genetics/mortality/pathology
EDAT- 2012/11/17 06:00
MHDA- 2013/03/05 06:00
CRDT- 2012/11/17 06:00
PHST- 2012/11/17 06:00 [entrez]
PHST- 2012/11/17 06:00 [pubmed]
PHST- 2013/03/05 06:00 [medline]
AID - 32/11/5031 [pii]
PST - ppublish
SO  - Anticancer Res. 2012 Nov;32(11):5031-7.